Identification of Th cell epitopes on clfA adhesin of Staphylococcus aureus and characterization of their role in immunity.

Abstract

OBJECTIVE:

The ClfA adhesin of Staphylococcus aureus is an excellent vaccine candidate antigen. CD4 + T cells play central roles during immune responses, but their functional contributions to Staphylococcus aureus in fection have yet to be evaluated.

METHODS:

By using the SYFPEITHI prediction algorithm, we identified and characterized four Th epitopes within the ClfA adhesin.

RESULTS:

Peptide C335 was I-Ed restricted Th1 type epitopes; peptides C214, C286, and C436 were I-Ad restricted Th2 type epitope.

CONCLUSION:

The identification of these epitopes is important to evaluate and optimize the vaccine-primed protection against Staphylococcus aureus infection.

• Authors: Yang Li, Zhijie Li, Yagang Li, Junfeng Ye
• Journal: Wei sheng wu xue bao = Acta microbiologica Sinica (Wei Sheng Wu Xue Bao) Vol. 53 Issue 9 Pg. 966-75 (Sep 04 2013) ISSN: 0001-6209 [Print] China
• PMID: 24377249 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adhesins, Bacterial
• ClfA protein, Staphylococcus aureus
• Coagulase
• Epitopes, T-Lymphocyte

Topics

• Adhesins, Bacterial (genetics, immunology)
• Amino Acid Sequence
• Animals
• CD4-Positive T-Lymphocytes (immunology)
• Coagulase (genetics, immunology)
• Epitopes, T-Lymphocyte (genetics, immunology)
• Female
• Humans
• Immunity
• Mice
• Mice, Inbred BALB C
• Molecular Sequence Data
• Staphylococcal Infections (immunology, microbiology)
• Staphylococcus aureus (enzymology, genetics, immunology)
• Th1 Cells (immunology)
• Th2 Cells (immunology)