Abstract | OBJECTIVE: METHODS: RESULTS: (1) The proliferation activity of 206m-group cell (0.74 ± 0.16) was significantly lower than that of control group (1.12 ± 0.23) (t = -3.066, P = 0.037) while that of 206i-group cell (1.43 ± 0.26) was higher than that of control group (0.98 ± 0.14) (t = 3.635, P = 0.022). (2) Transwell tests showed the migration and invasion of 206m-group cell decreased significantly (migration:0.56 ± 0.01 vs 0.63 ± 0.01, t = -23.00, P = 0.002; invasion:0.79 ± 0.01 vs 0.99 ± 0.01, t = -21.200, P = 0.002), but that of 206i-group cell increased significantly (migration:0.97 ± 0.11 vs 0.61 ± 0.09, t = 32.787, P = 0.001; invasion:1.10 ± 0.01 vs 0.93 ± 0.05, t = 5.167, P = 0.035). (3) The expressions of MMP-2,MMP-9 and Cx43 decreased and the expression of BRMS-1 increased in 206m-group cell and vice versa in 206i group. (4) The expression of miR-206 in lymph node-negative group of clinical breast cancer sample was higher than that of lymph node-positive one. And there was statistical difference (Z = -2.098, P = 0.003). And the expression of Cx43 was opposite. (5) Dual luciferase reporter assay confirmed the specific binding sites of hsa-miR-206 and Cx43. CONCLUSION:
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Authors | Yun Fu, Bei-qi Jiang, Yi Wu, Zheng-dong Li, Zhi-gang Zhuang |
Journal | Zhonghua yi xue za zhi
(Zhonghua Yi Xue Za Zhi)
Vol. 93
Issue 36
Pg. 2890-4
(Sep 24 2013)
ISSN: 0376-2491 [Print] China |
PMID | 24373402
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- BRMS1 protein, human
- Connexin 43
- MIRN206 microRNA, human
- MicroRNAs
- Neoplasm Proteins
- Repressor Proteins
- MMP2 protein, human
- Matrix Metalloproteinase 2
- MMP9 protein, human
- Matrix Metalloproteinase 9
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Topics |
- Breast Neoplasms
(genetics, metabolism, pathology)
- Cell Line, Tumor
- Cell Movement
- Cell Proliferation
- Connexin 43
(metabolism)
- Female
- Humans
- Matrix Metalloproteinase 2
(metabolism)
- Matrix Metalloproteinase 9
(metabolism)
- MicroRNAs
(genetics)
- Neoplasm Proteins
(metabolism)
- Repressor Proteins
- Transfection
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