Fibroblast growth factor 2 involved in the pathogenesis of synovial chondromatosis of temporomandibular joint.

Abstract

BACKGROUND:

Synovial chondromatosis (SC) of temporomandibular joint (TMJ) is a rare proliferative disorder characterized by the formation of cartilaginous or osteocartilaginous nodules in synovium and joint space. Fibroblast growth factor 2 (FGF-2) is frequently applied in chondrogenic differentiation assays. Therefore, we hypothesized that FGF-2 might involved in the pathogenesis of SC.

METHODS:

SC synovium and loose bodies (LBs) specimens were observed by histological and immunohistochemical methods. Real-time PCR was conducted for comparing genes expressions in SC and normal synovium. SC synoviocytes were stimulated by FGF-2 in the presence or absence of its antagonist long pentraxin-3 (PTX3) for 6 days. Real-time PCR and alkaline phosphatase (ALP) activity were performed to examine the effects exerted by FGF-2 and PTX3.

RESULTS:

SC synovium, no matter facing the articular cavity or covering LB, was characterized by increased quantity of synoviocytes and blood vessels. FGF-2 was expressed in chondrocytes and fibroblast-like cells of LBs, and the wall of blood vessels. Expressions of chondrogenic genes (Sox9 and Wnt-4), osteogenic genes (Foxc2), FGF-2, and VEGF-A mRNA were significantly higher in SC synovium than that of the control group. The stimulation of FGF-2 on SC synoviocytes increased ALP activity and expressions of chondrogenic genes (Sox9, Col2α1, and Aggrecan), osteogenic genes (Foxc2, osteocalcin, and Col1α1), and VEGF-A, but PTX3 inhibited these effects.

CONCLUSION:

FGF-2 was responsible for the formation of cartilaginous loose bodies and involved in the pathogenesis of SC.

• Authors: Yingjie Li, Hengxing Cai, Wei Fang, Qinggong Meng, Jian Li, Mohong Deng, Xing Long
• Journal: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology (J Oral Pathol Med) Vol. 43 Issue 5 Pg. 388-94 (May 2014) ISSN: 1600-0714 [Electronic] Denmark
• PMID: 24372705 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Chemical References

• Acute-Phase Proteins
• Aggrecans
• COL2A1 protein, human
• Collagen Type I
• Collagen Type I, alpha 1 Chain
• Collagen Type II
• Forkhead Transcription Factors
• SOX9 Transcription Factor
• SOX9 protein, human
• Serum Amyloid P-Component
• VEGFA protein, human
• Vascular Endothelial Growth Factor A
• WNT4 protein, human
• Wnt4 Protein
• mesenchyme fork head 1 protein
• Fibroblast Growth Factor 2
• Osteocalcin
• PTX3 protein
• C-Reactive Protein
• Alkaline Phosphatase

Topics

• Acute-Phase Proteins (pharmacology)
• Aggrecans (analysis)
• Alkaline Phosphatase (analysis)
• Blood Vessels (chemistry)
• C-Reactive Protein (pharmacology)
• Cell Culture Techniques
• Cells, Cultured
• Chondrocytes (chemistry)
• Chondrogenesis (drug effects)
• Chondromatosis, Synovial (etiology, metabolism)
• Collagen Type I (analysis)
• Collagen Type I, alpha 1 Chain
• Collagen Type II (analysis)
• Fibroblast Growth Factor 2 (analysis, antagonists & inhibitors, pharmacology)
• Fibroblasts (chemistry)
• Forkhead Transcription Factors (analysis)
• Humans
• Joint Loose Bodies (etiology, metabolism)
• Osteocalcin (analysis)
• Osteogenesis (drug effects)
• SOX9 Transcription Factor (analysis)
• Serum Amyloid P-Component (pharmacology)
• Synovial Membrane (chemistry, drug effects, pathology)
• Temporomandibular Joint Disorders (etiology, metabolism)
• Vascular Endothelial Growth Factor A (analysis)
• Wnt4 Protein (analysis)