[Effect of curcumin on expressions of mitogen-activated protein kinases and matrix metalloproteinases in Jurkat cells].

Abstract

OBJECTIVE:

To investigate the expressions of mitogen-activated protein kinases (MAPKs) and matrix metalloproteinases (MMPs) in apoptotic human T cell lymphoma Jurkat cells induced by curcumin in vitro and explore the possible molecular mechanisms of curcumin-induced apoptosis.

METHODS:

Jurkat cells were treated with different concentrations of curcumin, and the cell proliferation and cell cycle changes were detected by MTT assay and flow cytometry, respectively. Western blotting and gelatin zymography were employed to examine the protein expression levels of MAPKs and MMPs activity in the exposed cells.

RESULTS:

Curcumin inhibited the proliferation of Jurkat cells in a time- and dose-dependent manner, and caused cell cycle arrest in G0/G1 phase. Treatment of Jurkat cells with 25, 50, and 75 µmol/L curcumin resulted in a concentration-dependent increase of JNK and p-JNK expressions (P<0.01) without significantly affecting the expressions of ERK1/2 and P38 MAPK or the activity of MMP-2 and MMP-9.

CONCLUSION:

Curcumin within the concentration range of 6.25-25.00 µmol/L can induce apoptosis and cell cycle arrest of Jurkat cells, the mechanism of which might involve the activation of JNK pathway but not the MMPs.

• Authors: Guohua Zhu, Qi Zhang, Haiping Dai, Qun Shen
• Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (Nan Fang Yi Ke Da Xue Xue Bao) Vol. 33 Issue 12 Pg. 1792-5 (Dec 2013) ISSN: 1673-4254 [Print] China
• PMID: 24369247 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Mitogen-Activated Protein Kinase 3
• Mitogen-Activated Protein Kinases
• p38 Mitogen-Activated Protein Kinases
• Matrix Metalloproteinases
• MMP2 protein, human
• Matrix Metalloproteinase 2
• MMP9 protein, human
• Matrix Metalloproteinase 9
• Curcumin

Topics

• Apoptosis
• Cell Cycle
• Cell Division
• Cell Proliferation
• Curcumin (pharmacology)
• Flow Cytometry
• Humans
• Jurkat Cells
• MAP Kinase Signaling System
• Matrix Metalloproteinase 2 (metabolism)
• Matrix Metalloproteinase 9 (metabolism)
• Matrix Metalloproteinases (metabolism)
• Mitogen-Activated Protein Kinase 3 (metabolism)
• Mitogen-Activated Protein Kinases (metabolism)
• p38 Mitogen-Activated Protein Kinases (metabolism)