Abstract | PURPOSE: Nonequilibrium atmospheric pressure plasma (NEAPP) therapy has recently been focused on as a novel medical practice. Using cells with acquired paclitaxel/cisplatin resistance, we elucidated effects of indirect NEAPP-activated medium (NEAPP-AM) exposure on cell viability and tumor growth in vitro and in vivo. METHODS: RESULTS: We assessed the viability of NOS2 and NOS3 cells exposed to NEAPP-AM, which was prepared beforehand by irradiation with NEAPP for the indicated time. In NOS2 cells, viability decreased by approximately 30% after NEAPP-AM 120-sec treatment (P<0.01). The growth-inhibitory effects of NEAPP-AM were completely inhibited by N-acetyl cysteine treatment, while L- buthionine-[S, R]-sulfoximine, an inhibitor of the ROS scavenger used with NEAPP-AM, decreased cell viability by 85% after NEAPP-AM 60-sec treatment(P<0.05) and by 52% after 120 sec, compared to the control (P<0.01). In the murine subcutaneous tumor-formation model, NEAPP-AM injection resulted in an average inhibition of the NOS2 cell-inoculated tumor by 66% (P<0.05) and NOS2TR cell-inoculated tumor by 52% (P<0.05), as compared with the control. CONCLUSION: We demonstrated that plasma-activated medium also had an anti- tumor effect on chemo-resistant cells in vitro and in vivo. Indirect plasma therapy is a promising treatment option for EOC and may contribute to a better patient prognosis in the future.
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Authors | Fumi Utsumi, Hiroaki Kajiyama, Kae Nakamura, Hiromasa Tanaka, Masaaki Mizuno, Kenji Ishikawa, Hiroki Kondo, Hiroyuki Kano, Masaru Hori, Fumitaka Kikkawa |
Journal | PloS one
(PLoS One)
Vol. 8
Issue 12
Pg. e81576
( 2013)
ISSN: 1932-6203 [Electronic] United States |
PMID | 24367486
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Plasma Gases
- Taxoids
- Paclitaxel
- Cisplatin
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Topics |
- Animals
- Antineoplastic Combined Chemotherapy Protocols
(therapeutic use)
- Apoptosis
(drug effects)
- Atmospheric Pressure
- Cell Line, Tumor
- Cell Survival
(drug effects)
- Cisplatin
(therapeutic use)
- Drug Resistance, Neoplasm
- Female
- Humans
- Mice
- Mice, Nude
- Ovarian Neoplasms
(drug therapy)
- Paclitaxel
- Plasma Gases
(therapeutic use)
- Taxoids
(therapeutic use)
- Xenograft Model Antitumor Assays
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