Abstract | BACKGROUND AIMS: In a previous study, we successfully devised a prenatal surgical approach and transplanted mesenchymal stromal cells (MSCs) to fetal rat spinal column to treat retinoic acid-induced neural tube defects in rat. Our results show that MSCs survived, migrated and differentiated into neural lineage cells. We intended to study various growth factor expressions in rat fetal spinal cords with spina bifida aperta after in utero MSC transplantation and the effect of in vivo growth factor introduction for prenatal spina bifida treatment. METHODS: RESULTS: CONCLUSIONS: Our results suggest that intrinsic EGF and FGF-2, FGF-8 and FGF-20 might affect the in vivo fate of transplanted MSCs in a fetal rat spina bifida model. In vivo EGF introduction together with MSC transplantation might serve as a new strategy for prenatal spina bifida treatment.
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Authors | Hui Li, Jianing Miao, Guifeng Zhao, Di Wu, Bo Liu, Xiaowei Wei, Songying Cao, Hui Gu, Yi Zhang, Lili Wang, Yang Fan, Zhengwei Yuan |
Journal | Cytotherapy
(Cytotherapy)
Vol. 16
Issue 3
Pg. 319-30
(Mar 2014)
ISSN: 1477-2566 [Electronic] England |
PMID | 24364908
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 International Society for Cellular Therapy. All rights reserved. |
Chemical References |
- Tretinoin
- Fibroblast Growth Factors
- Epidermal Growth Factor
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Topics |
- Animals
- Apoptosis
(drug effects)
- Epidermal Growth Factor
(genetics, metabolism)
- Female
- Fetus
(physiology, surgery)
- Fibroblast Growth Factors
(genetics, metabolism)
- Genetic Vectors
(genetics)
- Lentivirus
(genetics)
- Mesenchymal Stem Cell Transplantation
- Pregnancy
- Rats
- Rats, Wistar
- Spina Bifida Cystica
(therapy)
- Spine
(physiology, surgery)
- Transcriptome
- Transgenes
(genetics)
- Tretinoin
(administration & dosage)
- Uterus
(surgery)
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