[Inhibitory effect of NF-kappaB p65siRNA on human laryngeal carcinoma xenograft model in nude mice].

Abstract

OBJECTIVE:

To investigate the inhibitory effect of NF-kappaB p65siRNA on human laryngeal carcinoma xenograft model in nude mice.

METHOD:

Human laryngeal carcinoma cell line Hep-2 was seeded in the subcutaneous layer of 15 nude mice to build laryngeal carcinoma xenograft model. Then they were randomly divided into three groups. NF-kappaB p65siRNA was given in siRNA group and FAM-Control siRNA was given in negative control group while phosphoric-buffered saline (PBS) was used in normal control group for 3 weeks. Tumor size and body weight of the mice were measured. TUNEL method and immunohistochemical S-P method were used for detecting the expression of NF-kappaB p65 and Bcl-xL protein.

RESULT:

The volume of tumors in siRNA group was reduced and the average weight of tumors in siRNA group was lower than the other two groups (P < 0.05). In siRNA group, the expression of NF-kappaB p65 and Bcl-xL protein was down-regulated and the apoptotic rate was increased obviously as compared with the negative control group and the normal control group.

CONCLUSION:

NF-kappaB p65siRNA can significantly inhibit the expression of NF-kappaB p65 and the growth of human laryngeal carcinoma xenograft in nude mice. Its mechanism may be related to inducing the apoptosis in tumor cells by down-regulating the expression of Bcl-xL protein.

• Authors: Hongjun Jin, Weihua Lou, Jianzhong Sang
• Journal: Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery (Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi) Vol. 27 Issue 15 Pg. 836-8 (Aug 2013) ISSN: 2096-7993 [Print] China
• PMID: 24364112 (Publication Type: English Abstract, Journal Article)

Chemical References

• RNA, Small Interfering
• Transcription Factor RelA
• bcl-X Protein

Topics

• Animals
• Apoptosis
• Carcinoma, Squamous Cell (genetics, pathology)
• Cell Line, Tumor
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Laryngeal Neoplasms (genetics, pathology)
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• RNA, Small Interfering
• Transcription Factor RelA (genetics)
• Xenograft Model Antitumor Assays
• bcl-X Protein (metabolism)