In vitro effect of genistein on DNA damage in leukocytes from mucopolysaccharidosis IVA patients.

Abstract

Mucopolysaccharidosis IVA is a lysosomal storage disorder leading to an increase in glycosaminoglycans storage. Genistein is an isoflavone capable to inhibit glycosaminoglycans production. The objective of this study was to analyze the in vitro effect of different concentrations of genistein on DNA injury in mucopolysaccharidosis IVA patients. The lower concentration tested (10 μM) showed a significant increase on DNA injury in vitro, although higher concentrations (30 μM and 50 μM) showed higher DNA damage.

• Authors: G W Negretto, M Deon, M Burin, G B Biancini, G Ribas, S C Garcia, G Goethel, R Fracasso, L Giugliani, R Giugliani, C R Vargas
• Journal: Molecular genetics and metabolism (Mol Genet Metab) Vol. 111 Issue 2 Pg. 205-8 (Feb 2014) ISSN: 1096-7206 [Electronic] United States
• PMID: 24359664 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Inc. All rights reserved.

Chemical References

• Genistein

Topics

• Adolescent
• Adult
• Cells, Cultured
• Child
• Comet Assay
• DNA Fragmentation (drug effects)
• Dose-Response Relationship, Drug
• Female
• Genistein (pharmacology)
• Humans
• Leukocytes, Mononuclear (drug effects, pathology)
• Male
• Mucopolysaccharidosis IV (pathology)