Pro-resolution mediator lipoxin A4 and its receptor in upper airway inflammation.

Abstract

OBJECTIVES:

The resolution of inflammation is an active process controlled by several anti-inflammatory and pro-resolution mediators. Lipoxin A4, an endogenous lipid mediator, is a potential pro-resolution mediator that could attenuate inflammation. This study was conducted to elucidate the role of lipoxin A4 in upper airway inflammation.

METHODS:

Nasal secretions were collected from patients with chronic rhinosinusitis with nasal polyposis, patients with allergic rhinitis, and control subjects. The concentration of lipoxin A4 was measured by enzyme-linked immunosorbent assay. Nasal tissues were obtained from nasal polyps and inferior turbinates during endonasal surgery. The mRNA expressions of lipoxygenases (LOXs), lipoxin receptor (formyl peptide receptor-like 1; FPRL-1), and cysteinyl leukotriene type 1 receptor (CysLT1R) in nasal tissues were examined by reverse-transcription polymerase chain reaction. Tissue localization of FPRL-1 was determined by immunohistochemical staining. The in vitro effect of lipoxin A4 on airway epithelial cells was also examined.

RESULTS:

A significant concentration of lipoxin A4 was found in nasal secretions, and the concentration was increased in patients with allergic rhinitis. The mRNA expressions of 5-LOX, 15-LOX-1, FPRL-1, and CysLT1R were significantly greater in nasal polyps than in inferior turbinates. FPRL-1 was localized in nasal epithelial cells. Lipoxin A4 inhibited tumor necrosis factor alpha-induced interleukin 8 release from airway epithelial cells via its receptor FPRL-1.

CONCLUSIONS:

These results indicate that lipoxin A4 may play a role in the resolution of upper airway inflammation. A low concentration of lipoxin A4 may be involved in chronic inflammation of the upper airways.

• Authors: Shino Shimizu, Takao Ogawa, Satoshi Seno, Hideaki Kouzaki, Takeshi Shimizu
• Journal: The Annals of otology, rhinology, and laryngology (Ann Otol Rhinol Laryngol) Vol. 122 Issue 11 Pg. 683-9 (Nov 2013) ISSN: 0003-4894 [Print] United States
• PMID: 24358628 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Eicosanoids
• FPR2 protein, human
• Lipoxins
• RNA, Messenger
• Receptors, Formyl Peptide
• Receptors, Lipoxin
• lipoxin A4

Topics

• Adult
• Aged
• Cells, Cultured
• Eicosanoids
• Enzyme-Linked Immunosorbent Assay
• Female
• Gene Expression Regulation
• Humans
• Immunohistochemistry
• Lipoxins (biosynthesis, genetics)
• Male
• Middle Aged
• Nasal Mucosa (metabolism, pathology)
• Nasal Polyps (genetics, metabolism)
• RNA, Messenger (genetics)
• Receptors, Formyl Peptide (biosynthesis, genetics)
• Receptors, Lipoxin (biosynthesis, genetics)
• Reverse Transcriptase Polymerase Chain Reaction
• Rhinitis (genetics, metabolism, pathology)
• Sinusitis (genetics, metabolism, pathology)