Hizikia fusiformis fractions successfully improve atopic dermatitis indices in anti-CD3-stimulated splenocytes and 2,4-dinitrochlorobenzene-treated BALB/c mice.

Abstract

OBJECTIVES:

In the present study, we aimed to examine whether fractions from an edible sea weed, Hizikia fusiformis, had immunomodulatory effects, particularly an anti-atopic effect, by attenuating the expression of T cell-dependent cytokines using in-vitro and in-vivo animal atopic dermatitis-like models.

METHODS:

The anti-atopic activities were examined in in vitro, and a 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis-like mouse model using quantitative real-time polymerase chain reaction, electrophoretic-mobility shift and histopathological analysis.

KEY FINDINGS:

Our results showed that the final fraction (F2') of H. fusiformis contained a higher amount of butanoic acid which was not found in the other fractions, and effectively inhibited T cell activation by inhibiting dephosphorylation of nuclear factor of activated T cells in electrophoretic-mobility shift assay. As a consequence, helper T cell-dependent cytokines, such as interleukin-2, -4 and interferon-γ, were significantly inhibited while activated with an anti-CD3 antibody. We also showed that skin challenged with DNCB successfully recovered when treated with 2.5 mg/kg, comparable to that by 0.25% prednicarbate. These results indicate that F2' may contribute to inhibit T cell activation by eliminating Th cell-dependent cytokines.

CONCLUSIONS:

Taken together, we concluded that F2' containing butanoic acid may be a new functional anti-atopic candidate, which probably acts through nuclear factor of activated T cell inactivation mechanisms.

• Authors: Kyu Ho Lee, Hee Jung Kim, Hae Bok Kim, Seung Tae Kim, Young Ri Choi, Da Woom Seo, Jung Min Yu, Su Kil Jang, Sang Moo Kim, Do-Ik Lee, Seong Soo Joo
• Journal: The Journal of pharmacy and pharmacology (J Pharm Pharmacol) Vol. 66 Issue 3 Pg. 466-76 (Mar 2014) ISSN: 2042-7158 [Electronic] England
• PMID: 24354475 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2013 Royal Pharmaceutical Society.

Chemical References

• Antibodies
• CD3 Complex
• Cytokines
• Dinitrochlorobenzene
• Immunologic Factors
• NFATC Transcription Factors
• Plant Extracts
• Butyric Acid

Topics

• Animals
• Antibodies (blood)
• Butyric Acid (analysis, pharmacology, therapeutic use)
• CD3 Complex (metabolism)
• Cytokines (metabolism)
• Dermatitis, Atopic (chemically induced, drug therapy, immunology, metabolism)
• Dinitrochlorobenzene
• Disease Models, Animal
• Immunologic Factors (pharmacology, therapeutic use)
• Lymphocyte Activation (drug effects)
• Male
• Mice
• Mice, Inbred BALB C
• NFATC Transcription Factors (metabolism)
• Phosphorylation
• Phytotherapy
• Plant Extracts (pharmacology, therapeutic use)
• Seaweed (chemistry)
• Skin (drug effects)
• T-Lymphocytes, Helper-Inducer (metabolism)