EGFR mutations in surgically resected fresh specimens from 697 consecutive Chinese patients with non-small cell lung cancer and their relationships with clinical features.

Abstract

We aimed to reveal the true status of epidermal growth factor receptor (EGFR) mutations in Chinese patients with non-small cell lung cancer (NSCLC) after lung resections. EGFR mutations of surgically resected fresh tumor samples from 697 Chinese NSCLC patients were analyzed by Amplification Refractory Mutation System (ARMS). Correlations between EGFR mutation hotspots and clinical features were also explored. Of the 697 NSCLC patients, 235 (33.7%) patients had tyrosine kinase inhibitor (TKIs) sensitive EGFR mutations in 41 (14.5%) of the 282 squamous carcinomas, 155 (52.9%) of the 293 adenocarcinomas, 34 (39.5%) of the 86 adenosquamous carcinomas, one (9.1%) of the 11 large-cell carcinomas, 2 (11.1%) of the 18 sarcomatoid carcinomas, and 2 (28.6%) of the 7 mucoepidermoid carcinomas. TKIs sensitive EGFR mutations were more frequently found in female patients (p < 0.001), non-smokers (p = 0.047) and adenocarcinomas (p < 0.001). The rates of exon 19 deletion mutation (19-del), exon 21 L858R point mutation (L858R), exon 21 L861Q point mutation (L861Q), exon 18 G719X point mutations (G719X, including G719C, G719S, G719A) were 43.4%, 48.1%, 1.7% and 6.8%, respectively. Exon 20 T790M point mutation (T790M) was detected in 3 squamous carcinomas and 3 adenocarcinomas and exon 20 insertion mutation (20-ins) was detected in 2 patients with adenocarcinoma. Our results show the rates of EGFR mutations are higher in all types of NSCLC in Chinese patients. 19-del and L858R are two of the more frequent mutations. EGFR mutation detection should be performed as a routine postoperative examination in Chinese NSCLC patients.

• Authors: Yuanyang Lai, Zhipei Zhang, Jianzhong Li, Dong Sun, Yong'an Zhou, Tao Jiang, Yong Han, Lijun Huang, Yifang Zhu, Xiaofei Li, Xiaolong Yan
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 14 Issue 12 Pg. 24549-59 (Dec 17 2013) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 24351833 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Protein Kinase Inhibitors
• EGFR protein, human
• ErbB Receptors

Topics

• Adenocarcinoma (drug therapy, genetics, surgery)
• Adult
• Aged
• Carcinoma, Non-Small-Cell Lung (drug therapy, genetics, pathology, surgery)
• Carcinoma, Squamous Cell (drug therapy, genetics, surgery)
• China
• ErbB Receptors (genetics, metabolism)
• Female
• Humans
• Lung Neoplasms (genetics, metabolism, pathology, surgery)
• Male
• Middle Aged
• Mutation
• Protein Kinase Inhibitors (therapeutic use)
• Smoking