Abstract | BACKGROUND: RESULTS: We have investigated the formation of dityrosine cross-links in Aβ42 formed by covalent ortho-ortho coupling of two tyrosine residues under conditions of oxidative stress with elevated copper and shown that dityrosine can be formed in vitro in Aβ oligomers and fibrils and that these links further stabilize the fibrils. Dityrosine crosslinking was present in internalized Aβ in cell cultures treated with oligomeric Aβ42 using a specific antibody for dityrosine by immunogold labeling transmission electron microscopy. Results also revealed the prevalence of dityrosine crosslinks in amyloid plaques in brain tissue and in cerebrospinal fluid from AD patients. CONCLUSIONS:
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Authors | Youssra K Al-Hilaly, Thomas L Williams, Maris Stewart-Parker, Lenzie Ford, Eldhose Skaria, Michael Cole, William Grant Bucher, Kyle L Morris, Alaa Abdul Sada, Julian R Thorpe, Louise C Serpell |
Journal | Acta neuropathologica communications
(Acta Neuropathol Commun)
Vol. 1
Pg. 83
(Dec 18 2013)
ISSN: 2051-5960 [Electronic] England |
PMID | 24351276
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Amyloid beta-Peptides
- Biomarkers
- Peptide Fragments
- amyloid beta-protein (1-42)
- Tyrosine
- Copper
- dityrosine
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Topics |
- Aged
- Aged, 80 and over
- Alzheimer Disease
(metabolism, pathology)
- Amyloid beta-Peptides
(metabolism)
- Biomarkers
(metabolism)
- Cells, Cultured
- Copper
(metabolism)
- Female
- Humans
- Male
- Neuroblastoma
- Oxidative Stress
(physiology)
- Peptide Fragments
(metabolism)
- Plaque, Amyloid
(metabolism, pathology)
- Tyrosine
(analogs & derivatives, metabolism)
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