Dynamic hyperpolarized carbon-13 MR metabolic imaging of nonhuman primate brain.

Abstract

PURPOSE:

To investigate hyperpolarized (13) C metabolic imaging methods in the primate brain that can be translated into future clinical trials for patients with brain cancer.

METHODS:

(13) C coils and pulse sequences designed for use in humans were tested in phantoms. Dynamic (13) C data were obtained from a healthy cynomolgus monkey brain using the optimized (13) C coils and pulse sequences. The metabolite kinetics were estimated from two-dimensional localized (13) C dynamic imaging data from the nonhuman primate brain.

RESULTS:

Pyruvate and lactate signal were observed in both the brain and the surrounding tissues with the maximum signal-to-noise ratio of 218 and 29 for pyruvate and lactate, respectively. Apparent rate constants for the conversion of pyruvate to lactate and the ratio of lactate to pyruvate showed a difference between brain and surrounding tissues.

CONCLUSION:

The feasibility of using hyperpolarized [1-(13) C]-pyruvate for assessing in vivo metabolism in a healthy nonhuman primate brain was demonstrated using a hyperpolarized (13) C imaging experimental setup designed for studying patients with brain tumors. The kinetics of the metabolite conversion suggests that this approach may be useful in future studies of human neuropathology.

• Authors: Ilwoo Park, Peder E Z Larson, James L Tropp, Lucas Carvajal, Galen Reed, Robert Bok, Fraser Robb, John Bringas, Adrian Kells, Philip Pivirotto, Krystof Bankiewicz, Daniel B Vigneron, Sarah J Nelson
• Journal: Magnetic resonance in medicine (Magn Reson Med) Vol. 71 Issue 1 Pg. 19-25 (Jan 2014) ISSN: 1522-2594 [Electronic] United States
• PMID: 24346964 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Wiley Periodicals, Inc.

Chemical References

• Carbon Isotopes
• Lactic Acid
• Pyruvic Acid

Topics

• Animals
• Brain (anatomy & histology, metabolism)
• Carbon Isotopes (pharmacokinetics)
• Equipment Design
• Equipment Failure Analysis
• Feasibility Studies
• Female
• Humans
• Lactic Acid (metabolism)
• Macaca fascicularis
• Magnetic Resonance Imaging (instrumentation, methods)
• Magnetic Resonance Spectroscopy (instrumentation, methods)
• Pyruvic Acid (metabolism)
• Reproducibility of Results
• Sensitivity and Specificity