The efficiency of the correction of combined alimentary
vitamin deficit in male Wistar rats (
body weight 90-121 g) fed standard diet or enriched with omega-3
polyunsaturated fatty acids diet (by replacing
sunflower oil (4.5% of the diet) with
linseed oil) with different doses (physiological and enhanced) of
vitamins has been investigated. The control group of animals (n = 12) received a complete semisynthetic diet during all experimental period (42 d). The animals of the test groups (each of 20 rats) received
vitamin-deficient diet containing sunflower or
linseed oil and 20% per cent of
vitamin mixture amount in control diet from which
vitamin E had been excluded. After 28 days of such feeding each of test groups was divided into two subgroups (6-8 rats in each), and the next 14 days the animals of subgroups received the diet with different degree of
vitamin compensation (50 or 180% percent of
vitamin content in the diet of the control group). The addition of both low and high
vitamin dose in deficient diet based on standard fat component did not compensate the reduced liver
vitamin A content, which amounted to 47.4% of the level in the liver of the control group. The lack of
vitamin E in animals was eliminated only after adding of the enhanced dose of
vitamin E to the ration. Recovering of decreased plasma and liver B2 level, plasma 25(
OH)D and liver
vitamin B1 content have been occurred after addition of the low dose of these
vitamins to rat diet. Increasing of omega-3 PUFA diet level improved
vitamins A and D sufficiency to some extent, but was accompanied by the significant reduction of rat liver alfa-
tocopherol content both under combined
vitamin deficiency (by 14%) and increased
vitamins consumption (by 43%). PUFA enrichment of the diet of rats with
vitamin deficiency had no impact on
vitamin B1 and B2 liver level. The use of high doses of
vitamins for a long time to eliminate a combined deficiency of
vitamins has been proved.