Abstract |
Cell-transfer technique has been proven useful for performing immunocytochemistry on fine-needle aspiration smears. However, its utility for EGFR and KRAS molecular testing has not been validated. Molecular testing was performed using the cell-transfer technique on both Papanicolaou-stained ethanol-fixed and Hema 3-stained air-dried smears from 32 fine-needle aspiration samples that had diagnoses of adenocarcinoma of the lung, and then was compared to the results of the corresponding formalin-fixed paraffin-embedded tissues. The molecular testing was successfully performed on 32 of 32 ethanol-fixed and 31 of 32 air-dried samples. The molecular results on ethanol-fixed and air-dried smears showed 100% agreement. There is 100% (32/32) agreement for the EGFR and 97% (31/32) agreement for the KRAS between the cell-transfer technique and formalin-fixed paraffin-embedded tissues. One discrepant case was due to low percentage of tumor cells on the smears. Cell-transfer technique is a reliable alternative method for EGFR and KRAS testing if the cell blocks lack adequate cellularity.
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Authors | Howard H Wu, Joseph P Eaton, Kelly J Jones, Harvey M Cramer, Melissa L Randolph, Kristin M Post, Amanda Malek, Sarah Bilbo, Joyashree D Sen, Shaoxiong Chen, Liang Cheng |
Journal | Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
(Mod Pathol)
Vol. 27
Issue 7
Pg. 930-5
(Jul 2014)
ISSN: 1530-0285 [Electronic] United States |
PMID | 24336155
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- KRAS protein, human
- Proto-Oncogene Proteins
- EGFR protein, human
- ErbB Receptors
- Proto-Oncogene Proteins p21(ras)
- ras Proteins
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Topics |
- Adenocarcinoma
(diagnosis, genetics, pathology)
- Adult
- Aged
- Aged, 80 and over
- DNA Mutational Analysis
(methods)
- ErbB Receptors
(genetics)
- Female
- Humans
- Lung
(pathology)
- Lung Neoplasms
(diagnosis, genetics, pathology)
- Male
- Middle Aged
- Proto-Oncogene Proteins
(genetics)
- Proto-Oncogene Proteins p21(ras)
- ras Proteins
(genetics)
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