Distribution of mast cells and macrophages and expression of interleukin-6 in periapical cysts.

Abstract

INTRODUCTION:

Mast cells and macrophages are important components of the inflammatory infiltrate found in inflammatory periapical diseases. Several cytokines participate in the mechanisms of inflammation, tissue repair, and bone resorption associated with periapical cysts. The aim of the present study was to evaluate the distribution of mast cells and macrophages and the expression of interleukin-6 (IL-6) in periapical cysts.

METHODS:

Thirty periapical cysts were selected for the study, and clinical, demographic, and gross information from the cases was obtained from the laboratory records. Five-micrometer sections stained with hematoxylin-eosin were reviewed for analysis of the microscopic features of the cysts, and 3-μm sections on silanized slides were used for immunohistochemical reactions with anti-tryptase, anti-CD68, and anti-IL-6.

RESULTS:

There was no statistically significant difference in the mean number of mast cells and macrophages when comparing superficial and deep regions of the fibrous capsule of the cysts. Mean number of mast cells on the superficial region of the fibrous capsule was higher in cysts showing intense superficial inflammation and exocytosis. Macrophages were more commonly found in areas showing IL-6 expression, and IL-6 was less expressed in deep regions of the fibrous capsule in cysts showing greater gross volume.

CONCLUSIONS:

The results reinforced the participation of mast cells and macrophages in the pathogenesis of periapical cysts and suggested that IL-6 is not the major bone resorption mediator in larger periapical cysts.

• Authors: Igor Vieira Bracks, Luciana Armada, Lúcio Souza Gonçalves, Fábio Ramôa Pires
• Journal: Journal of endodontics (J Endod) Vol. 40 Issue 1 Pg. 63-8 (Jan 2014) ISSN: 1878-3554 [Electronic] United States
• PMID: 24331993 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

Chemical References

• Antigens, CD
• Antigens, Differentiation, Myelomonocytic
• CD68 antigen, human
• IL6 protein, human
• Interleukin-6
• Cholesterol
• Tryptases

Topics

• Adult
• Aged
• Antigens, CD (analysis)
• Antigens, Differentiation, Myelomonocytic (analysis)
• Cell Count
• Cholesterol (analysis)
• Connective Tissue (immunology, pathology)
• Epithelium (immunology, pathology)
• Exocytosis (immunology)
• Female
• Humans
• Interleukin-6 (analysis)
• Macrophages (immunology, pathology)
• Male
• Mast Cells (immunology, pathology)
• Middle Aged
• Radicular Cyst (immunology, pathology)
• Tryptases (analysis)
• Young Adult