Abstract |
Severe meconium aspiration syndrome (MAS) in newborns is often treated by exogenous surfactant. Because its efficacy is reduced by meconium-induced inflammation, glucocorticoid budesonide was added into surfactant preparation Curosurf to enhance efficacy of the surfactant therapy in experimental model of MAS. Oxygen-ventilated rabbits were intratracheally given meconium (25 mg/ml, 4 ml/kg) to induce respiratory failure. Thirty minutes later, animals were treated by intratracheal budesonide (0.25 mg/kg) or surfactant lung lavage (10 ml/kg, 5 mg phospholipids/ml) repeated twice, followed by undiluted Curosurf (100 mg phospholipids/kg) or by the above mentioned surfactant treatment with the last surfactant dose fortified with budesonide (0.25 mg/kg) or were untreated. Animals were ventilated for additional 5 hours and respiratory parameters were measured regularly. After sacrificing animals, wet-dry lung weight ratio was evaluated and plasma levels of interleukins (IL)-1beta, -6, -8, and TNF-alpha were measured by ELISA method. Efficacy of the given therapies to enhance lung functions and to diminish lung edema formation and inflammation increased from budesonide-only and surfactant-only therapy to surfactant+budesonide therapy. Combined therapy improved gas exchange from 30 min of administration, and showed a longer-lasting effect than surfactant-only therapy. In conclusions, budesonide additionally improved the effects of exogenous surfactant in experimental MAS.
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Authors | P Mikolka, D Mokrá, J Kopincová, L Tomčíková-Mikušiaková, A Calkovská |
Journal | Physiological research
(Physiol Res)
Vol. 62
Issue Suppl 1
Pg. S191-200
( 2013)
ISSN: 1802-9973 [Electronic] Czech Republic |
PMID | 24329699
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-Inflammatory Agents
- Biological Products
- Bronchodilator Agents
- Drug Combinations
- Immunologic Factors
- Phospholipids
- Pulmonary Surfactants
- Budesonide
- poractant alfa
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Topics |
- Animals
- Anti-Inflammatory Agents
(administration & dosage)
- Biological Products
(administration & dosage)
- Bronchodilator Agents
(administration & dosage)
- Budesonide
(administration & dosage)
- Drug Combinations
- Drug Synergism
- Female
- Immunologic Factors
(metabolism)
- Male
- Meconium Aspiration Syndrome
(diagnosis, drug therapy, physiopathology)
- Phospholipids
(administration & dosage)
- Pulmonary Surfactants
- Rabbits
- Respiratory Mechanics
(drug effects)
- Swine
- Treatment Outcome
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