Resveratrol suppresses inflammatory responses in endometrial stromal cells derived from endometriosis: a possible role of the sirtuin 1 pathway.

Abstract

AIM:

Endometriosis is a chronic inflammatory disease. Sirtuin 1 (SIRT1) plays a role in regulation of inflammation. The role of SIRT1 in endometriosis remains unknown. We here addressed the anti-inflammatory effects of SIRT1 on endometriosis.

METHODS:

The expression of SIRT1 in human ovarian endometriomas and eutopic endometria were examined using immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR). Endometriotic stromal cells (ESC) obtained from endometriomas were exposed to either resveratrol or sirtinol, an activator or inhibitor of sirtuins, respectively, and tumor necrosis factor (TNF)-α-induced interleukin (IL)-8 release from the ESC was assessed at mRNA and protein levels.

RESULTS:

Both immunochemistry and RT-PCR demonstrated that SIRT1 was expressed in ESC and normal endometrial stromal cells. Resveratrol suppressed TNF-α-induced IL-8 release from the ESC in a dose-dependent manner while sirtinol increased IL-8 release.

CONCLUSION:

These opposing effects of SIRT1-related agents suggest that IL-8 release from the ESC is modulated through the SIRT1 pathway. Resveratrol may have the potential to ameliorate local inflammation in endometriomas.

• Authors: Ayumi Taguchi, Osamu Wada-Hiraike, Kei Kawana, Kaori Koga, Aki Yamashita, Akira Shirane, Yoko Urata, Shiro Kozuma, Yutaka Osuga, Tomoyuki Fujii
• Journal: The journal of obstetrics and gynaecology research (J Obstet Gynaecol Res) Vol. 40 Issue 3 Pg. 770-8 (Mar 2014) ISSN: 1447-0756 [Electronic] Australia
• PMID: 24320086 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2013 The Authors. Journal of Obstetrics and Gynaecology Research © 2013 Japan Society of Obstetrics and Gynecology.

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Benzamides
• CXCL8 protein, human
• Enzyme Activators
• Enzyme Inhibitors
• Interleukin-8
• Naphthols
• Stilbenes
• sirtinol
• SIRT1 protein, human
• Sirtuin 1
• Resveratrol

Topics

• Adult
• Anti-Inflammatory Agents, Non-Steroidal (pharmacology)
• Benzamides (pharmacology)
• Cells, Cultured
• Endometriosis (drug therapy, immunology, metabolism, pathology)
• Endometrium (drug effects, immunology, metabolism, pathology)
• Enzyme Activators (pharmacology)
• Enzyme Inhibitors (pharmacology)
• Female
• Humans
• Interleukin-8 (metabolism)
• Naphthols (pharmacology)
• Ovarian Diseases (drug therapy, immunology, metabolism, pathology)
• Ovary (drug effects, immunology, metabolism, pathology)
• Resveratrol
• Signal Transduction (drug effects)
• Sirtuin 1 (antagonists & inhibitors, chemistry, genetics, metabolism)
• Stilbenes (pharmacology)
• Stromal Cells (drug effects, immunology, metabolism, pathology)