Tucaresol down-modulation of MUC1-stimulated human mononuclear cells.

Abstract

Interaction between antigen presenting cells and T lymphocytes, through receptors and co-stimulatory molecules present on the surface of these cells, is one of the key means to modulate the adaptive immune system. Tucaresol, a Schiff-base-forming chemical, can be used as a substitute for the physiological donor of carbonyl groups of antigen presenting cells, which can interact with the amine groups of T lymphocytes to modulate the adaptive immune system. This study was done to determine whether tucaresol can enhance killing of cancer cells in vitro as well as protect non-obese diabetic severe combined immunodeficient mice from tumor development by mucin 1 stimulated human mononuclear cells through the adaptive immune system. The expected hypothesis was not supported. Percent specific lysis of MCF-7 tumor cells by mucin 1 stimulated human mononuclear cells was reduced by tucaresol. Furthermore, tucaresol abolished the protective effect of mucin 1 stimulated human mononuclear cells against MCF-7 breast cancer cell growth in non-obese diabetic severe combined immunodeficient mice. This study implies that tucaresol may be of use as an immunosuppressive agent.

• Authors: Stephen E Wright, Kathleen A Rewers-Felkins, Nazrul I Chowdhury, Jewel Ahmed, Sanjay K Srivastava, Pamela R Lockwood-Cooke
• Journal: Immunological investigations (Immunol Invest) Vol. 43 Issue 2 Pg. 160-9 ( 2014) ISSN: 1532-4311 [Electronic] England
• PMID: 24303799 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Antigens, Neoplasm
• Benzaldehydes
• Benzoates
• Mucin-1
• tucaresol

Topics

• Adenocarcinoma (immunology, therapy)
• Animals
• Antigen-Presenting Cells (drug effects, immunology)
• Antigens, Neoplasm (immunology)
• Benzaldehydes (administration & dosage)
• Benzoates (administration & dosage)
• Breast Neoplasms (immunology, therapy)
• Cytotoxicity, Immunologic (drug effects)
• Humans
• Immunological Synapses (metabolism)
• Immunosuppression Therapy
• Leukocytes, Mononuclear (immunology)
• Lymphocyte Activation (drug effects)
• MCF-7 Cells
• Mice
• Mice, Inbred NOD
• Mice, SCID
• Mucin-1 (immunology)
• Protein Carbonylation
• T-Lymphocytes (immunology)
• Xenograft Model Antitumor Assays