Magnesium lithospermate B improves myocardial function and prevents simulated ischemia/reperfusion injury-induced H9c2 cardiomyocytes apoptosis through Akt-dependent pathway.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Magnesium lithospermate B (MLB), an active polyphenol acid of Radix Salviae Miltiorrhizae (Danshen), showed a wide range of pharmacological activities in cardiovascular diseases. However, its role in protection against ischemia/reperfusion injury in H9c2 cardiomyocytes has not been elucidated. This study was aimed to investigate the protective effect and potential molecular mechanisms of MLB on apoptosis in H9c2 cardiomyocytes in vitro.

MATERIALS AND METHODS:

We tested cell viability, shortening amplitude, necrosis, apoptosis, and the expression levels of Akt, phosphorylated Akt, Bcl-2 and Bax after 2-h simulated ischemia and 24-h simulated reperfusion in H9c2 cardiomyocytes. We further observed the contractile function in hearts after they were subjected to global 30-min ischemia and 180-min reperfusion.

RESULTS:

Pretreatment with MLB markedly increased cell viability and while reducing evidence of necrosis and apoptosis in H9c2 cardiomyocytes. In addition, the expression of Bcl-2 and Bax protein was modulated. The results also showed that MLB significantly increased phosphorylation of Akt and that this phosphorylation can be partially inhibited by phosphoinositide 3-kinase/Akt inhibitor. Furthermore, MLB improved MI/R-induced myocardial contractile function.

CONCLUSION:

Our results showed that MLB prevents I/R-induced myocardial damage by reducing necrosis and apoptosis in H9c2 cardiomyocytes and improving myocardial function in rat hearts.

• Authors: Wei Quan, Bin Wu, Yan Bai, Xiaohong Zhang, Jipeng Yin, Miaomiao Xi, Yue Guan, Qing Shao, Yichen Chen, Qiangju Wu, Aidong Wen
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 151 Issue 1 Pg. 714-21 ( 2014) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 24296090 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2013 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Drugs, Chinese Herbal
• lithospermate B
• Proto-Oncogene Proteins c-akt

Topics

• Animals
• Apoptosis (drug effects)
• Cell Line
• Drugs, Chinese Herbal (pharmacology)
• Gene Expression Regulation (drug effects)
• Male
• Molecular Structure
• Myocytes, Cardiac (cytology, drug effects, metabolism)
• Proto-Oncogene Proteins c-akt (genetics, metabolism)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (prevention & control)
• Signal Transduction