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Effect of cyclin G2 on proliferative ability of prostate cancer PC-3 cell.

Abstract
This study aimed to analyze the expression, clinical significance of cyclin G2 (CCNG2) in prostate carcinoma, and the biological effect in its cell line by CCNG2 overexpression. Immunohistochemistry and Western blot were used to analyze CCNG2 protein expression in 85 cases of prostate cancer and normal tissues to study the relationship between CCNG2 expression and clinical factors. CCNG2 lentiviral vector and empty vector were, respectively, transfected into prostate cancer PC-3 cell line. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were used to detect the mRNA level and protein of CCNG2. MTT assay and cell cycle were also conducted as to the influence of the upregulated expression of CCNG2 that might be found on PC-3 cells biological effect. The level of CCNG2 protein expression was found to be significantly lower in prostate cancer tissue than normal tissues (P < 0.05). The level of CCNG2 protein expression was not correlated with age, PSA contention, and tumor size (P < 0.05), but it was correlated with lymph node metastasis, clinic stage, and Gleason score (P < 0.05). The result of biological function shown that PC-3 cell transfected CCNG2 had a lower survival fraction, more percentage of the G0/G1 phases, and lower CDK2 protein expression compared with PC-3 cell untransfected CCNG2 (P < 0.05). CCNG2 expression decreased in prostate cancer and correlated significantly with lymph node metastasis, clinic stage, and Gleason score, suggesting that CCNG2 may play important roles as a negative regulator to prostate cancer cell.
AuthorsD W Cui, Y J Cheng, S W Jing, G G Sun
JournalTumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (Tumour Biol) Vol. 35 Issue 4 Pg. 3017-24 (Apr 2014) ISSN: 1423-0380 [Electronic] Netherlands
PMID24293374 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • CCNG2 protein, human
  • Cyclin G2
  • Protein Phosphatase 2
Topics
  • Cell Line, Tumor
  • Cell Proliferation
  • Cyclin G2 (analysis, physiology)
  • Humans
  • Lymphatic Metastasis
  • Male
  • Neoplasm Staging
  • Prostatic Neoplasms (chemistry, pathology)
  • Protein Phosphatase 2 (physiology)

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