The clinicopathological significance of lamin A/C, lamin B1 and lamin B receptor mRNA expression in human breast cancer.

Abstract

Lamin A/C (LMNA), lamin B1 (LMNB1) and lamin B receptor (LBR) have key roles in nuclear structural integrity and chromosomal stability. In this study, we have studied the relationships between the mRNA expressions of A-type lamins, LMNB1 and LBR and the clinicopathological parameters in human breast cancer. Samples of breast cancer tissues (n = 115) and associated non-cancerous tissue (ANCT; n = 30) were assessed using reverse transcription and quantitative PCR. Transcript levels were correlated with clinicopathological data. Higher levels of A-type lamins and LMNB1 mRNA expression were seen in ANCT. Higher lamin A/C expression was associated with the early clinical stage (TNM1 vs. TNM3 - 13 vs. 0.21; p = 0.0515), with better clinical outcomes (disease-free survival vs. mortality - 11 vs. 1; p = 0.0326), and with better overall (p = 0.004) and disease-free survival (p = 0.062). The expression of LMNB1 declined with worsening clinical outcome (disease-free vs. mortalities - 0.0011 vs. 0.000; p = 0.0177). LBR mRNA expression was directly associated with tumor grade (grade 1 vs. grade 3 - 0.00 vs. 0.00; p = 0.0479) and Nottingham Prognostic Index (NPI1 vs. NPI3 - 0.00 vs. 0.00; p = 0.0551). To the best of our knowledge, this is the first study to suggest such a role for A-type lamins, lamin B1 and LBR in human breast cancer, identifying an important area for further research.

• Authors: Umar Wazir, Mai Hassan Ahmed, Joanna M Bridger, Amanda Harvey, Wen G Jiang, Anup K Sharma, Kefah Mokbel
• Journal: Cellular & molecular biology letters (Cell Mol Biol Lett) Vol. 18 Issue 4 Pg. 595-611 (Dec 2013) ISSN: 1689-1392 [Electronic] England
• PMID: 24293108 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Lamin Type A
• Lamin Type B
• RNA, Messenger
• Receptors, Cytoplasmic and Nuclear
• lamin B receptor
• lamin B1

Topics

• Breast (metabolism, pathology)
• Breast Neoplasms (genetics, pathology)
• Cell Cycle
• Chromosomal Instability
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Lamin Type A (genetics)
• Lamin Type B (genetics)
• RNA, Messenger (genetics)
• Receptors, Cytoplasmic and Nuclear (genetics)