Antiallergic effects of pigments isolated from green sea urchin (Strongylocentrotus droebachiensis) shells.

Abstract

This study was undertaken to evaluate possible antiallergic effects of an extract of pigments from green sea urchin (Strongylocentrotus droebachiensis) shells. Effects were studied on animal models - guinea pig ileum contraction, rabbit eyes allergic conjunctivitis, and rabbit local skin irritation. The extract significantly reduced, in a dose-dependent manner, the histamine-induced contractions of the isolated guinea pig ileum with ID50 =1.2 µg/mL (in equivalents of spinochrome B), had an inhibitory effect on the model of ocular allergic inflammation surpassing the reference drug olopatadine, and did not show any irritating effect in rabbits. The extract predominantly contained polyhydroxy-1,4-naphthoquinone which would be responsible for the pharmacological activity. The active compounds of the extract were evaluated in silico with molecular docking. Molecular docking into H1R receptor structures obtained from molecular dynamic simulations showed that all spinochrome derivatives bind to the receptor active site, but spinochrome monomers fit better to it. The results of the present study suggest possibilities for the development of new agents for treating allergic diseases on the base of pigments from sea urchins shells.

• Authors: Olga N Pozharitskaya, Alexander N Shikov, Marina N Makarova, Svetlana A Ivanova, Vera M Kosman, Valery G Makarov, Václav Bazgier, Karel Berka, Michal Otyepka, Jitka Ulrichová
• Journal: Planta medica (Planta Med) Vol. 79 Issue 18 Pg. 1698-704 (Dec 2013) ISSN: 1439-0221 [Electronic] Germany
• PMID: 24288292 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Georg Thieme Verlag KG Stuttgart · New York.

Chemical References

• Anti-Allergic Agents
• Dibenzoxepins
• Naphthoquinones
• Pigments, Biological
• Olopatadine Hydrochloride
• spinochrome A
• Histamine
• 1,4-naphthoquinone

Topics

• Animal Shells (chemistry)
• Animals
• Anti-Allergic Agents (chemistry, isolation & purification, pharmacology)
• Conjunctivitis, Allergic (drug therapy)
• Dibenzoxepins (pharmacology)
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Guinea Pigs
• Histamine (pharmacology)
• Ileum (drug effects)
• Male
• Molecular Docking Simulation
• Naphthoquinones (chemistry, isolation & purification, pharmacology)
• Olopatadine Hydrochloride
• Pigments, Biological (chemistry)
• Rabbits
• Skin (drug effects)
• Strongylocentrotus (chemistry)