Abstract |
The effect of 1-oleoyl-2-acetyl-glycerol (OAG) and the phorbol diester 12-O-tetradecanoyl-phorbol-acetate (TPA) on the intracellular Ca2+ concentration ([Ca2+]i) in NG108-15 cells was studied using a Ca2+ indicator, quin 2. OAG and TPA induced an increase in [Ca2+]i from 100 +/- 19 to 187 +/- 24 nM and 192 +/- 15 nM, respectively, within 15 min. The increase in [Ca2+]i induced by activators of protein kinase C was dependent on the extracellular Ca2+ concentration [Ca2+]o) and was inhibited by the Ca2+ blockers, verapamil and nifedipine. These results indicate that the OAG- and TPA-induced [Ca2+]i increase is mediated by the influx of extracellular Ca2+ through voltage-sensitive Ca2+ channels.
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Authors | T Osugi, T Imaizumi, A Mizushima, S Uchida, H Yoshida |
Journal | European journal of pharmacology
(Eur J Pharmacol)
Vol. 126
Issue 1-2
Pg. 47-51
(Jul 15 1986)
ISSN: 0014-2999 [Print] Netherlands |
PMID | 2428631
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Diglycerides
- Glycerides
- Ion Channels
- 1-oleoyl-2-acetylglycerol
- Tetradecanoylphorbol Acetate
- Calcium
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Topics |
- Calcium
(metabolism)
- Diglycerides
(pharmacology)
- Glioma
(metabolism)
- Glycerides
(pharmacology)
- Humans
- Hybrid Cells
- Ion Channels
(drug effects)
- Kinetics
- Nervous System Neoplasms
(metabolism)
- Neuroblastoma
(metabolism)
- Tetradecanoylphorbol Acetate
(pharmacology)
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