Abstract |
The rapid degradation of porcine glucagon-like peptide-2 (pGLP-2) by the enzyme dipeptidyl peptidase-IV (DPP-IV) is the main impediment in the development of pGLP-2 as a potential therapeutic agent for intestinal dysfunction and damage. In this study, one mono-modified Lys(30)-polyethylene glycol (PEG)-pGLP-2 was prepared using mPEG-succinimidyl propionate. To determine the optimized condition for PEGylation, the reactions were monitored by RP-HPLC and MALDI-TOF-MS. Stability was tested in purified DPP-IV in vitro. In vivo, the protective effects for colonic injury were measured in dextran sulfate sodium (DSS)-induced colitis in mice. The monoPEGylated products reached the maximum yield at 4:1 ratio of mPEG5k-SPA to pGLP-2. An effective method of successfully separating PEGylated pGLP-2 from mPEG-SPA5kD using CM Sepharose Fast Flow resin was established. The half-life of Lys(30)-PEG-pGLP-2 was 16-fold longer than that of pGLP-2 in DPP-IV. The DSS mice exhibited marked weight loss), which was significantly reduced by Lys(30)-PEG-pGLP-2 therapy. DSS treatment significantly increased colonic damage score, which was significantly reduced by administration of Lys(30)-PEG-pGLP-2 in DSS-mice. DSS-induced colitis clearly induced Myeloperoxidase activity in the colon, which was significantly reduced by treatments with 3% DSS-pGLP-2 or 3% DSS-PEG-pGLP-2. These results showed that site-specific Lys(30)-PEG-GLP-2 was resistant to degradation and reduced the severity of colonic injury in murine colitis. The enhanced biological potency of this product highlighted its potential as a therapeutic agent for intestinal diseases.
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Authors | Ke-ke Qi, Jie Wu, Jing Wan, Xiao-ming Men, Zi-wei Xu |
Journal | Peptides
(Peptides)
Vol. 52
Pg. 11-8
(Feb 2014)
ISSN: 1873-5169 [Electronic] United States |
PMID | 24274971
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 Elsevier Inc. All rights reserved. |
Chemical References |
- Glucagon-Like Peptide 2
- Polyethylene Glycols
- Dextran Sulfate
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Topics |
- Animals
- Chronic Disease
- Colitis
(chemically induced, drug therapy, mortality, pathology)
- Colon
(metabolism, pathology)
- Dextran Sulfate
(toxicity)
- Disease Models, Animal
- Glucagon-Like Peptide 2
(chemistry, pharmacology)
- Male
- Mice
- Mice, Inbred BALB C
- Polyethylene Glycols
(chemistry, pharmacology)
- Swine
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