The aim of this study was to identify new
protein markers of the intestinal and diffuse type gastric
adenocarcinoma and to determine their relation to local relapses and distant
metastasis. Using two-dimensional gel electrophoresis, we searched for
proteins that are overexpressed in the intestinal and/or diffuse type gastric
adenocarcinoma, as compared to matched normal mucosa samples with further change confirmation by Western blot. Expression of the selected
proteins was further assessed by immunohistocemistry in a large panel of gastric
adenocarcinoma with various clinicopathological features. Expression level of
cyclophilin A measured with western blot appeared to be increased on average ten times in 63 % of gastric
adenocarcinoma vs. paired samples of normal mucosa. The frequency of immunihistochemistry detected
cyclophilin A protein expression was found to be equal in
tumor of both histotypes, but staining intensity was higher in intestinal versus diffuse types of gastric
adenocarcinoma.
cyclophilin A protein expression appeared to be lower in deeply invading glandular and cribriform structures of intestinal
tumors, as well as in discretely placed groups of the intestinal
tumor cells. Local relapses as well as distant
metastases registered within 3 year follow up were observed to occur much less frequently in patients with positive
cyclophilin A immunostaining in gastric
tumors. Analysis of
cyclophilin A expression has a potential value for prognosis of gastric
adenocarcinoma recurrence and distant
metastasis.