This study aims to analyze the expression and clinical significance of
cyclin G2 (CCNG2) in kidney
carcinoma, and the
biological effect in its cell line by CCNG2 overexpression. Immunohistochemistry and western blot were used to analyze CCNG2
protein expression in 63 cases of
kidney cancer and normal tissues to study the relationship between CCNG2 expression and clinical factors. CCNG2 lentiviral vector and empty vector were respectively transfected into kidney ACHN cell line. During immunohistochemistry, the level of CCNG2
protein expression was found to be significantly lower in
kidney cancer tissue than normal tissues (P < 0.05). After Western blot, the relative amount of CCNG2
protein in
kidney cancer tissue was respectively found to be significantly lower than in normal tissues (P < 0.05). The level of CCNG2
protein expression was not correlated with gender, age,
tumor size, and pathological types (P > 0.05), but it was correlated with
lymph node metastasis, clinic stage, and histological grade (P < 0.05). Loss of CCNG2 expression correlated significantly with poor overall survival time by Kaplan-Meier analysis (P < 0.05). The result of
biological function show that ACHN cell-transfected CCNG2 had a lower survival fraction, higher percentage of the G0/G1 phases, and lower
CDK2 protein expression compared with ACHN cell-untransfected CCNG2 (P < 0.05). CCNG2 expression decreased in
kidney cancer and correlated significantly with
lymph node metastasis, clinical stage, histological grade, and poor overall survival, suggesting that CCNG2 may play important roles as a negative regulator to
kidney cancer ACHN cell by promoting degradation of CDK2.