Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: MATERIALS AND METHODS: We extracted ZDW with artificial intestinal fluid and treated rat renal tubular cells (NRK-52E) with various concentrations of the ZDW extraction. Cell proliferation and gentamicin-induced apoptosis of NRK-52E cells were evaluated using real-time proliferation monitoring and annexin V staining, respectively. Western blotting was used to evaluate the levels of Bcl-2 and caspase-3 expression. The effect of ZDW on gentamicin-induced kidney injury was also monitored in mice using the terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL) assay, and the measurement of serum creatinine and blood urea nitrogen. RESULTS: We found that 30 μg/ml of ZDW promoted cell proliferation of the rat renal tubular cells. ZDW also expressed a dose-dependent protective effect against gentamicin-induced apoptosis in the cells. Pretreatment with 3 μg/ml or 30 μg/ml of ZDW maximally increased Bcl-2 and decreased cleaved caspase-3 in the gentamicin-treated NRK-52E cells. Among the herbal ingredients of ZDW, only Phellodendron amurense Rupr., bark (Cortex Phellodendri), and Anemarrhena asphodeloides Bunge, rhizome inhibited both the gentamicin-induced Bcl-2 decrease and cleaved caspase-3 increase. Phellodendron amurense Rupr., bark and Anemarrhena asphodeloides Bunge, rhizome also inhibited gentamicin-induced apoptosis at particular concentrations; however, these two ingredients were less effective than ZDW. In the mouse model of gentamicin-induced nephropathy, the ZDW treatment significantly reduced apoptotic cells in the renal cortex and improved renal function. CONCLUSIONS: Our results suggest that ZDW at adequate doses attenuates gentamicin-induced apoptotic injury in renal tubular cells and also protects kidneys from gentamicin-induced injury in mice.
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Authors | Yung-Ho Hsu, Tso-Hsiao Chen, Mei-Yi Wu, Yuh-Feng Lin, Wen-Liang Chen, Tzu-Hurng Cheng, Cheng-Hsien Chen |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 151
Issue 1
Pg. 635-42
( 2014)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 24269250
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2013 Published by Elsevier Ireland Ltd. |
Chemical References |
- Drugs, Chinese Herbal
- Gentamicins
- Protein Synthesis Inhibitors
- zhibai dihuang
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Topics |
- Animals
- Apoptosis
(drug effects)
- Cell Line
- Cell Proliferation
- Drugs, Chinese Herbal
(therapeutic use)
- Gene Expression Regulation
(drug effects)
- Gentamicins
(toxicity)
- Kidney Diseases
(chemically induced, drug therapy)
- Kidney Tubules
(cytology)
- Male
- Mice
- Mice, Inbred BALB C
- Protein Synthesis Inhibitors
(toxicity)
- Rats
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