Abstract |
Epimedin C, an ingredient of Herba Epimedii, has potential for treatment of cardiovascular disease and bone loss. However, there is still no sensitive analytical method to monitor epimedin C in biological samples. The goal of this study was to develop a sensitive and reliable method based on a LC-MS/MS for evaluating the pharmacokinetics of epimedin C after administration of Herba Epimedii in rat. Electrospray ionization in positive-ion mode and multiple reaction monitoring were used to identify and quantitate active components. Analytes were separated by a reverse-phase C18 column. Liquid-liquid extraction using ethyl acetate, evaporation and reconstitution was used to plasma sample preparation. Mass transition of precursor ion → product ion pairs were monitored at m/z 823.4 → 313.1 for epimedin C and m/z 237.1 → 178.9 for carbamazepine (internal standard). A calibration curve gave good linearity (r > 0.999) over the concentration range 2.5-500 ng/mL. Pharmacokinetic data demonstrated that there was rapid distribution and slow elimination after epimedin C administration (1 mg/kg, i.v.). Oral bioavailabilities of epimedin C in the pure compound and in the Herba Epimedii were around 0.58% and 0.13%, respectively. The result suggests that other herbal ingredients of Herba Epimedii may suppress the oral bioavailability of epimedin C.
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Authors | Chia-Jung Lee, Yu-Tse Wu, Thomas Y Hsueh, Lie-Chwen Lin, Tung-Hu Tsai |
Journal | Biomedical chromatography : BMC
(Biomed Chromatogr)
Vol. 28
Issue 5
Pg. 630-6
(May 2014)
ISSN: 1099-0801 [Electronic] England |
PMID | 24264996
(Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 John Wiley & Sons, Ltd. |
Chemical References |
- Drugs, Chinese Herbal
- Flavonoids
- epimedii flavone
- epimedin C
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Topics |
- Administration, Oral
- Animals
- Biological Availability
- Chromatography, High Pressure Liquid
(methods)
- Drugs, Chinese Herbal
(administration & dosage, chemistry, pharmacokinetics)
- Flavonoids
(administration & dosage, pharmacokinetics)
- Male
- Rats
- Rats, Sprague-Dawley
- Tandem Mass Spectrometry
(methods)
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