Plakoglobin represses SATB1 expression and decreases in vitro proliferation, migration and invasion.

Abstract

Plakoglobin (γ-catenin) is a homolog of β-catenin with dual adhesive and signaling functions. Plakoglobin participates in cell-cell adhesion as a component of the adherens junction and desmosomes whereas its signaling function is mediated by its interactions with various intracellular protein partners. To determine the role of plakoglobin during tumorigenesis and metastasis, we expressed plakoglobin in the human tongue squamous cell carcinoma (SCC9) cells and compared the mRNA profiles of parental SCC9 cells and their plakoglobin-expressing transfectants (SCC9-PG). We observed that the mRNA levels of SATB1, the oncogenic chromatin remodeling factor, were decreased approximately 3-fold in SCC9-PG cells compared to parental SCC9 cells. Here, we showed that plakoglobin decreased levels of SATB1 mRNA and protein in SCC9-PG cells and that plakoglobin and p53 associated with the SATB1 promoter. Plakoglobin expression also resulted in decreased SATB1 promoter activity. These results were confirmed following plakoglobin expression in the very low plakoglobin expressing and invasive mammary carcinoma cell line MDA-MB-231 cells (MDA-231-PG). In addition, knockdown of endogenous plakoglobin in the non-invasive mammary carcinoma MCF-7 cells (MCF-7-shPG) resulted in increased SATB1 mRNA and protein. Plakoglobin expression also resulted in increased mRNA and protein levels of the metastasis suppressor Nm23-H1, a SATB1 target gene. Furthermore, the levels of various SATB1 target genes involved in tumorigenesis and metastasis were altered in MCF-7-shPG cells relative to parental MCF-7 cells. Finally, plakoglobin expression resulted in decreased in vitro proliferation, migration and invasion in different carcinoma cell lines. Together with the results of our previous studies, the data suggests that plakoglobin suppresses tumorigenesis and metastasis through the regulation of genes involved in these processes.

• Authors: Zackie Aktary, Manijeh Pasdar
• Journal: PloS one (PLoS One) Vol. 8 Issue 11 Pg. e78388 ( 2013) ISSN: 1932-6203 [Electronic] United States
• PMID: 24260116 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Desmoplakins
• JUP protein, human
• Matrix Attachment Region Binding Proteins
• Neoplasm Proteins
• SATB1 protein, human
• gamma Catenin

Topics

• Breast Neoplasms (genetics, metabolism, pathology)
• Cell Line, Tumor
• Cell Movement
• Cell Proliferation
• Desmoplakins (biosynthesis, genetics)
• Down-Regulation
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Matrix Attachment Region Binding Proteins (genetics, metabolism)
• Neoplasm Invasiveness (genetics, pathology)
• Neoplasm Proteins (genetics, metabolism)
• gamma Catenin