Silencing MAP3K1 expression through RNA interference enhances paclitaxel-induced cell cycle arrest in human breast cancer cells.

Abstract	The objective of this study is to compare the expression level of MAP3K1 between normal mammary gland cells and breast cancer cells, and to analyze the effects of silencing MAP3K1 on breast cancer cells with paclitaxel treatment. Western blotting analysis was used to detect the expression level of MAP3K1 in MCF-7 and MCF-12F cells. The effect of gene silencing through different siRNAs was determined by realtime-PCR. MTT assay was used to test the cell proliferation. Cell cycle was detected by flow cytometry. MAP3K1 protein expression level in breast cancer cells was higher than that in normal mammary gland cells. MAP3K1 siRNA transfection significantly reduced the expression level of MAP3K1, and enhanced paclitaxel-induced cell proliferation inhibition and cell cycle arrest in breast cancer cells. Targeting MAP3K1 expression through small RNA interference can promote the therapeutic effects of paclitaxel in breast cancer.
Authors	Pinghua Hu, Qin Huang, Zhihua Li, Xiaobo Wu, Qianwen Ouyang, Jun Chen, Yali Cao
Journal	Molecular biology reports (Mol Biol Rep) Vol. 41 Issue 1 Pg. 19-24 (Jan 2014) ISSN: 1573-4978 [Electronic] Netherlands
PMID	24253898 (Publication Type: Journal Article)
Chemical References	Antineoplastic Agents, Phytogenic CCNB1 protein, human Cyclin B1 RNA, Small Interfering MAP Kinase Kinase Kinase 1 MAP3K1 protein, human CDC25C protein, human cdc25 Phosphatases Paclitaxel
Topics	Antineoplastic Agents, Phytogenic (pharmacology) Breast Neoplasms Cell Cycle Checkpoints (drug effects) Cell Proliferation Cyclin B1 (genetics, metabolism) Female Gene Expression Gene Knockdown Techniques Humans MAP Kinase Kinase Kinase 1 (genetics, metabolism) MCF-7 Cells Mammary Glands, Human (enzymology, pathology) Paclitaxel (pharmacology) RNA Interference RNA, Small Interfering (genetics) cdc25 Phosphatases (genetics, metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!