Hypothyroidism has been associated to
psychiatric disorder development and tissue oxidative damage. In this study, we evaluated the effect of
diphenyl diselenide supplementation on depressive-like behavior triggered by
methimazole exposure in female rats. Additionally,
thiobarbituric acid reactive substances (
TBARS),
reactive oxygen species (ROS) and non-
protein thiol (NP-SH) levels were analyzed in cerebral cortex, hippocampus and striatum structures of rats.
Monoamine oxidase (
MAO) activity was evaluated in total brain. Firstly, female rats received
methimazole (MTZ) 20mg/100ml in the
drinking water for 30days and were evaluated in open-field and forced swimming tests (FST). In this set of experiments, the rats exposed to MTZ presented a depressive-like behavior, which was evidenced by a significant increase in the immobility time when compared to control group. Thereafter, MTZ-induced hypothyroid rats received either a standard or a diet containing 5ppm of
diphenyl diselenide, and then they were evaluated monthly in open-field and FST tests during 3months. No alteration on the locomotor performance was observed among the groups. The depressive-like behavior of hypothyroid rats was blunted by
diphenyl diselenide supplementation during all experimental periods. The levels of
thyroid hormones remained low in MTZ exposed groups until the end of experimental period. The MTZ group had an increase in
TBARS and ROS levels that were restored by
diphenyl diselenide supplementation. NP-SH content of cerebral structures was not modified by MTZ exposure and/or
diphenyl diselenide supplementation.
Diphenyl diselenide supplementation restored the
MAO B activity that was decreased in MTZ group. In summary, our results show that
hypothyroidism induced by MTZ
methimazole triggers a depressive-like behavior in female rats and that dietary
diphenyl diselenide was able to reduce this effect.