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Assessment of murine neuroblastoma (N1E-115) resting membrane potential by confocal microscopy.

Abstract
Digital imaging (confocal microscopy) and a slow potentiometric dye (tetramethylrhodamine methyl ester) were used to assess the resting membrane potential (V m) of murine neuroblastoma cells (N1E-115). The averageV m was found to be -64.0±2.0 mV. The difference between this and the previously reported higher values was attributed to the use of glass microelectrode techniques that probably caused mechanical injury to the cell membranes: Digital imaging of N1E-115V m was found to be sensitive, reproducible, fast, and simple.
AuthorsM Hernandez, W S Kisaalita, M A Farmer
JournalJournal of fluorescence (J Fluoresc) Vol. 6 Issue 2 Pg. 77-82 (Jun 1996) ISSN: 1053-0509 [Print] Netherlands
PMID24227081 (Publication Type: Journal Article)

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