Serological cross-reactions between Brucella abortus and Yersinia enterocolitica 0:9.

Abstract

Yersinia enterocolitica (Ye) 0:9 is an organism of great significance in veterinary medicine largely as a result of its cross-reaction with Brucella abortus (Ba). Boty Ye 0:9 and Ba possess somatic antigens in common; as a result of which animals exposed to Ye 0:9 have an immune response which is distinguishable only with difficulty from that induced by Ba. Cattle were exposed to Ye 0:9 by the oral or intramammary routes. Oral exposure failed to generate significant serologic response. In contrast, intramammary inoculation produced a marked response. Serum antibodies provoked in this manner reacted strongly with Ba. The anti-Brucella response provoked by inoculation of Yersinia was sufficient to render milk and serum Brucella-seropositive as measured by the standard milk ring and serum agglutination tests. While both Ba and Ye 0:9 have 9 antigens in common, they differ significantly with respect to motility. Thus Ba is always non motile while Ye is motile when grown at room temperature. The presence of Yersinia H agglutinins in serum were shown to be evidence of previous exposure to Ye. The H agglutinins were not generated by Brucella infection. A rapid H agglutination test was shown to provide this differentiation without interference from cross-reacting O antigens. Results of Ba O and Ye O and OH antigens used in the agglutination test were found useful to differentiate antibodies against Ba from those induced by Ye 0:9 in cattle sera. The existence of enterobacterial common antigen (ECA) in Ye and its absence in Ba were utilized in an attempt to provide a method to distinguish Brucella infections from those with cross-reacting Yersinia.(ABSTRACT TRUNCATED AT 250 WORDS)

• Authors: K R Mittal, I R Tizard, D A Barnum
• Journal: International journal of zoonoses (Int J Zoonoses) Vol. 12 Issue 3 Pg. 219-27 (Sep 1985) ISSN: 0377-0168 [Print] China (Republic : 1949- )
• PMID: 2422134 (Publication Type: Journal Article)

Chemical References

• Antibodies, Bacterial
• Antigens, Bacterial
• O Antigens
• enterobacterial common antigen

Topics

• Animals
• Antibodies, Bacterial (analysis, immunology)
• Antigens, Bacterial (immunology)
• Brucella abortus (immunology)
• Brucellosis, Bovine (diagnosis, immunology)
• Cattle
• Cattle Diseases (immunology, microbiology)
• Cross Reactions
• Diagnosis, Differential
• False Positive Reactions
• O Antigens
• Yersinia Infections (diagnosis, immunology, veterinary)
• Yersinia enterocolitica (immunology)