Abstract | BACKGROUND AIMS: Mesenchymal stromal cells (MSCs) exhibit the inherent potential to regulate multiple signaling pathways and cell types that contribute to the pathogenesis of inflammatory and immune diseases. However, more recent studies have suggested that the secretion of immunomodulatory factors by MSCs can be enhanced by three-dimensional aggregation or pro-inflammatory cytokine treatment. METHODS: RESULTS: Culturing human MSCs as three-dimensional aggregates increased secretion of immunomodulatory paracrine factors, which was enhanced further by treatment with IFN-γ and TNF-α, demonstrating that these parameters can synergistically enhance endogenous human MSC immunomodulatory properties. However, immunomodulatory factor secretion was found to be highly dependent on the composition of cell culture medium. Human MSCs cultured in MesenCult-XF medium displayed significantly less expression of prostaglandin E2, indoleamine 2,3-dioxygenase, transforming growth factor-β1 and interleukin-6 compared with human MSCs cultured in medium supplemented with fetal bovine serum. Finally, pre-conditioning of human MSC spheroids with IFN-γ and TNF-α resulted in greater immunomodulatory activity in a macrophage co-culture assay. CONCLUSIONS: Altogether, engineering the environment of human MSCs to develop pre-conditioning strategies for enhancing human MSC immunomodulation may be a simple approach for improving MSC-based therapies for the treatment of inflammatory and immune diseases.
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Authors | Joshua A Zimmermann, Todd C McDevitt |
Journal | Cytotherapy
(Cytotherapy)
Vol. 16
Issue 3
Pg. 331-45
(Mar 2014)
ISSN: 1477-2566 [Electronic] England |
PMID | 24219905
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Culture Media
- Indoleamine-Pyrrole 2,3,-Dioxygenase
- Interleukin-6
- Transforming Growth Factor beta1
- Tumor Necrosis Factor-alpha
- Interferon-gamma
- Dinoprostone
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Topics |
- Cells, Cultured
- Coculture Techniques
- Culture Media
(chemistry)
- Dinoprostone
(metabolism)
- Humans
- Immunomodulation
- Indoleamine-Pyrrole 2,3,-Dioxygenase
(metabolism)
- Interferon-gamma
(immunology)
- Interleukin-6
(metabolism)
- Macrophage Activation
- Mesenchymal Stem Cell Transplantation
- Mesenchymal Stem Cells
(cytology, immunology, metabolism)
- Paracrine Communication
- Spheroids, Cellular
(cytology)
- Transforming Growth Factor beta1
(metabolism)
- Transplantation Conditioning
- Tumor Necrosis Factor-alpha
(immunology, metabolism)
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