Comparative evaluation of a new chemiluminiscent assay and an ELISA for the detection of IgM against measles.

Abstract

BACKGROUND:

The primary test for the laboratory confirmation of measles is immunoglobulin M (IgM) serology. It is therefore important to evaluate new commercial measles IgM immunoassays to ensure high-quality measles diagnostic testing. The purpose of this study was to evaluate the diagnostic performance of LIAISON IgM measles (DiaSorin, Saluggia, Italy), a new automated chemiluminescence immunoassay (CLIA), in comparison with enzyme immunoassay (EIA) Enzygnost (Siemens, Marburg, Germany).

METHODS:

Sensitivity was examined using a panel of measles laboratory confirmed sera; specificity was addressed by testing sera from healthy populations and subjects with infections that may interfere with measles IgM serology.

RESULTS:

The diagnostic performances of the two assays were very similar: both Enzygnost EIA and LIAISON CLIA performed with a sensitivity of 93.7% and 98.8%, whereas the specificity was 96.8% and 97.9%, respectively.

CONCLUSION:

We concluded that LIAISON IgM measles can be a good alternative to the other immunoassay for laboratory measles confirmation.

• Authors: Antonio Sampedro, Javier Rodríguez-Granger, Cristina Gómez, Ana Lara, José Gutierrez, Ana Otero
• Journal: Journal of clinical laboratory analysis (J Clin Lab Anal) Vol. 27 Issue 6 Pg. 477-80 (Nov 2013) ISSN: 1098-2825 [Electronic] United States
• PMID: 24218130 (Publication Type: Journal Article)
• Copyright: © 2013 Wiley Periodicals, Inc.

Chemical References

• Antibodies, Viral
• Immunoglobulin G

Topics

• Antibodies, Viral (blood)
• Enzyme-Linked Immunosorbent Assay (methods)
• Humans
• Immunoglobulin G (blood)
• Luminescent Measurements (methods)
• Measles (diagnosis, immunology, virology)
• Sensitivity and Specificity
• Virology (methods)