Abstract |
Caenorhabditis elegans, a cholesterol auxotroph, showed defects in larval development upon cholesterol starvation (CS) in a previous study. To identify cholesterol-responsive proteins likely responsible for the larval arrest upon CS, a comparative proteomic analysis was performed between C. elegans grown in normal medium supplemented with cholesterol (CN) and those grown in medium not supplemented with cholesterol ( cholesterol starvation, CS). Our analysis revealed significant change (more than 2.2-fold, p < 0.05) in nine proteins upon CS. Six proteins were down-regulated [CE01270 (EEF-1A.1), CE08852 (SAMS-1), CE11068 (PMT-2), CE09015 (ACDH-1), CE12564 (R07H5.8), and CE09655 (RLA-0)], and three proteins were up-regulated [CE29645 (LEC-1), CE16576 (LEC-5), and CE01431 (NEX-1)]. RNAi phenotypes of two of the down-regulated genes, R07H5.8 ( adenosine kinase) and rla-0 ( ribosomal protein), in CN were similar to that of larval arrest in CS, and RNAi of a down-regulated gene, R07H5.8, in CS further enhanced the effects of CS, suggesting that down-regulation of these genes is likely responsible for the larval arrest in CS. All three up-regulated genes contain putative DAF-16 binding sites and mRNA levels of these three genes were all decreased in daf-16 mutants in CN, suggesting that DAF-16 activates expression of these genes.
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Authors | Ichiro Kawasaki, Myung-Hwan Jeong, Yu-Joun Yun, Yun-Kyung Shin, Yhong-Hee Shim |
Journal | Molecules and cells
(Mol Cells)
Vol. 36
Issue 5
Pg. 410-6
(Nov 2013)
ISSN: 0219-1032 [Electronic] Korea (South) |
PMID | 24218109
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Annexins
- Caenorhabditis elegans Proteins
- Forkhead Transcription Factors
- Galectins
- Lec-1 protein, C elegans
- Nex-1 protein, C elegans
- Transcription Factors
- daf-16 protein, C elegans
- Cholesterol
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Topics |
- Animals
- Annexins
(metabolism)
- Binding Sites
- Caenorhabditis elegans
(genetics, growth & development, metabolism)
- Caenorhabditis elegans Proteins
(genetics, metabolism)
- Cholesterol
(metabolism)
- Down-Regulation
- Forkhead Transcription Factors
- Galectins
(metabolism)
- Gene Expression Regulation, Developmental
- Larva
(genetics, growth & development, metabolism)
- Methylation
- Phenotype
- Phosphorylation
- Proteomics
- Reverse Genetics
- Signal Transduction
(genetics)
- Transcription Factors
(metabolism)
- Up-Regulation
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