Abstract |
Astilbin, a flavonoid compound was isolated from the rhizome of Smilax china L. In this study, we investigated the anti- myocardial ischemia and reperfusion (I/R) injury effect of Astilbin on diabetic rats in vivo and elucidated the potential mechanism in vitro. The results showed that Astilbin significantly attenuated hypoxia-induced cell injury in a concentration-dependent manner. Treatment of H9c2 cells with Astilbin at 15 μM blocked nuclear factor kappaB (NF-κB) phosphorylation by blocking High-mobility group box protein 1 ( HMGB1) expression. Treatment of diabetic rats with Astilbin by intravenous injection (i.v.) at a single dose of 50 mg/kg protected the rats from myocardial I/R injury as indicated by decreasing infarct volume, improving hemodynamics and reducing myocardial damage, and also lowered serum levels of pro-inflammatory factors, reduced HMGB1 and phosphorylated NF-κB expression in ischemic myocardial tissue from diabetic rats. Additionally, treatment of diabetic rats with Astilbin at dose of 50 mg/kg by i.v. for continuous 14 days attenuated cardiac remodeling in the model myocardial I/R injury. These protective effects suggested that Astilbin might be due to block of the myocardial inflammatory cascade via the HMGB1-dependent NF-κB signaling pathway.
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Authors | Huiling Diao, ZeChun Kang, Fang Han, Wanglin Jiang |
Journal | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
(Food Chem Toxicol)
Vol. 63
Pg. 104-10
(Jan 2014)
ISSN: 1873-6351 [Electronic] England |
PMID | 24211745
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 Elsevier Ltd. All rights reserved. |
Chemical References |
- Flavonols
- HMGB1 Protein
- Hbp1 protein, rat
- Inflammation Mediators
- NF-kappa B
- astilbin
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Topics |
- Animals
- Blotting, Western
- Diabetes Mellitus, Experimental
(complications)
- Flavonols
(pharmacology)
- HMGB1 Protein
(physiology)
- Heart
(drug effects)
- Inflammation Mediators
(blood)
- Male
- Myocardial Reperfusion Injury
(prevention & control)
- NF-kappa B
(metabolism)
- Phosphorylation
- Rats
- Rats, Sprague-Dawley
- Signal Transduction
(drug effects)
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