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The neuroprotective effect of carnosine (β-alanyl-L-histidine) on retinal ganglion cell following ischemia-reperfusion injury.

AbstractPURPOSE:
To investigate whether carnosine can increase retinal ganglion cell (RGC) survival in ischemic mouse retina.
METHODS:
Retinal ischemia was induced by constant elevation of intraocular pressure (100-110 mmHg) for 60 min in C57BL/6 J mice pretreated with carnosine (1000 mg/kg) or saline. Hypoxia inducing factor-1 alpha (HIF-1α), glial fibrillary acidic protein (GFAP), and dynamin-related protein-1 (Drp-1) expressions were assessed at 6, 12, and 24 h after retinal ischemia. Bax and Bcl-2 expressions were also analyzed at 12 h after retinal ischemia. RGC survival was assessed by retrograde FluoroGold labeling at 2 weeks after retinal ischemia.
RESULTS:
The expression of HIF-1α, GFAP, and Drp-1 was increased within 24 h after ischemic injury. Carnosine treatment effectively decreased the elevated expression of HIF-1α, GFAP, and Drp-1 in ischemic mouse retina. In ischemic retina treated with carnosine, Bax expression was decreased, whereas Bcl-2 expression was increased compared with ischemic retina treated with saline. Carnosine treatment also protected against RGC loss in ischemia mouse retina.
CONCLUSIONS:
Our findings showed that carnosine treatment significantly decreased RGC loss through decreased expression of HIF-1α, GFAP, Drp-1, and Bax, and increased expression of Bcl-2 in ischemic mouse retina. We suggest that carnosine can be an effective endogenous neuroprotective molecule in the prevention of RGC loss in ischemic retina.
AuthorsYong-Sok Ji, Jung-Won Park, Hwan Heo, Jong-Seong Park, Sang-Woo Park
JournalCurrent eye research (Curr Eye Res) Vol. 39 Issue 6 Pg. 634-41 (Jun 2014) ISSN: 1460-2202 [Electronic] England
PMID24206188 (Publication Type: Journal Article)
Chemical References
  • Bax protein, mouse
  • Biomarkers
  • Hif1a protein, mouse
  • Hypoxia-Inducible Factor 1, alpha Subunit
  • Nerve Tissue Proteins
  • Neuroprotective Agents
  • Proto-Oncogene Proteins c-bcl-2
  • bcl-2-Associated X Protein
  • glial fibrillary astrocytic protein, mouse
  • Carnosine
  • Dnm1l protein, mouse
  • Dynamins
Topics
  • Animals
  • Biomarkers (metabolism)
  • Blotting, Western
  • Carnosine (administration & dosage, therapeutic use)
  • Cell Count
  • Cell Survival (drug effects)
  • Dynamins (metabolism)
  • Fluorescent Antibody Technique, Indirect
  • Hypoxia-Inducible Factor 1, alpha Subunit (metabolism)
  • Injections, Intraperitoneal
  • Mice
  • Mice, Inbred C57BL
  • Nerve Tissue Proteins (metabolism)
  • Neuroprotective Agents (administration & dosage, therapeutic use)
  • Proto-Oncogene Proteins c-bcl-2 (metabolism)
  • Reperfusion Injury (drug therapy)
  • Retinal Diseases (drug therapy, metabolism)
  • Retinal Ganglion Cells (drug effects, metabolism)
  • bcl-2-Associated X Protein (metabolism)

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