Reduction of the HIV seroconversion window period and false positive rate by using ADVIA Centaur HIV antigen/antibody combo assay.

Early diagnosis of HIV infection reduces morbidity and mortality. Fourth-generation HIV detection assays are more sensitive because they can detect p24 antigen as well as anti-HIV antibodies. In this study, we evaluated the performance of a new fourth-generation ADVIA Centaur HIV antigen/antibody combo (CHIV) assay (Siemens Healthcare Diagnostics Inc., USA) for early detection of HIV infection and reduction of false positive rate.
Four seroconversion panels were included. The third-generation ADVIA Centaur HIV 1/O/2 enhanced (EHIV) assay (Siemens Healthcare Diagnostics Inc., USA) and fourth-generation CHIV assay were used to test each panel for HIV infection. The presence of antigen was confirmed using HIV p24 antigen assay. To evaluate false-positivity and specificity, 54 HIV false-positive and HIV-negative serum samples from 100 hospitalized patients and 600 healthy subjects were included.
COMPARED TO THE EHIV ASSAY, THE CHIV ASSAY HAD A SHORTER WINDOW FOR THREE OF THE SEROCONVERSION PANELS: a difference of 10 days and two bleeds in one panel, and 4 days and one bleed in the other two panels. Only 34 of the 54 (63%) samples known to yield false-positive results by EHIV assay had repeatedly yielded reactive results in the CHIV assay. One of the 600 healthy subjects had a false-positive result with the CHIV assay; thus, the specificity was 99.85% (699/700). CHIV accurately determined the reactive results for the HIV-confirmed serum samples from known HIV patients and Korea Food & Drug Administration (KFDA) panels.
The new fourth-generation ADVIA Centaur HIV assay is a sensitive and specific assay that shortens the serological window period and allows early diagnosis of HIV infection.
AuthorsKyunghoon Lee, Hyung-Doo Park, Eun-Suk Kang
JournalAnnals of laboratory medicine (Ann Lab Med) Vol. 33 Issue 6 Pg. 420-5 (Nov 2013) ISSN: 2234-3814 [Electronic] Korea (South)
PMID24205491 (Publication Type: Comparative Study, Journal Article)
Chemical References
  • HIV Antibodies
  • HIV Core Protein p24
  • Reagent Kits, Diagnostic
  • False Positive Reactions
  • Female
  • HIV Antibodies (blood, immunology)
  • HIV Core Protein p24 (blood, immunology)
  • HIV Seropositivity (diagnosis)
  • Humans
  • Male
  • Pregnancy
  • Reagent Kits, Diagnostic
  • Republic of Korea
  • Sensitivity and Specificity
  • Time Factors

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