Abstract | OBJECTIVE: The aim of this study was to investigate whether the DOR agonist BW373U86 conferred neuroprotection following ACA when given after resuscitation and to determine the long-term effects of chronic BW373U86 treatment on ACA-elicited brain injury. METHODS: Animals were divided into acute and chronic treatment groups. Each group consisted of four sub-groups, including Sham, ACA, BW373U86 (BW373U86+ACA), and Naltrindole groups ( Naltrindole and BW373U86+ACA). The DOR antagonist Naltrindole was used to confirm the possible receptor-dependent effects of BW373U86. ACA was induced by 8min of asphyxiation followed by resuscitation. All drugs were administered either immediately after the restoration of spontaneous circulation (ROSC) in acute-treatment groups or over 6 consecutive days in chronic-treatment groups. Alterations of cAMP response element-binding protein (CREB) and phosphorylated CREB (pCREB) were analyzed by western blot and immunohistochemistry. Neurological functions were assessed by neurological deficit score ( NDS) and Morris Water Maze performance. Neurodegeneration was monitored by immunofluorescence and Nissl staining. RESULTS: ACA induced massive neuron loss and serious neurological function deficits. BW373U86 significantly reduced both of these negative effects and increased CREB and pCREB expression in the hippocampus; these effects were reversed with acute Naltrindole treatment. The protective effects of BW373U86 persisted until 28d post-ROSC with chronic treatment, but these effects were not reversed by Naltrindole. CONCLUSIONS:
BW373U86 attenuates global cerebral ischemic injury induced by ACA through both DOR-dependent and DOR-independent mechanisms. CREB might be an important molecule in mediating these neuroprotective effects.
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Authors | Lu Yang, Xiaoyong Zhao, Meiyan Sun, Xude Sun, Linong Yao, Daihua Yu, Qian Ding, Changjun Gao, Wei Chai |
Journal | Resuscitation
(Resuscitation)
Vol. 85
Issue 2
Pg. 299-305
(Feb 2014)
ISSN: 1873-1570 [Electronic] Ireland |
PMID | 24200890
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Benzamides
- Neuroprotective Agents
- Piperazines
- BW 373U86
- Naltrexone
- CREB-Binding Protein
- Crebbp protein, rat
- naltrindole
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Topics |
- Animals
- Asphyxia
(therapy)
- Benzamides
(pharmacology)
- Blotting, Western
- Brain Damage, Chronic
(metabolism, pathology, prevention & control)
- CREB-Binding Protein
(metabolism)
- Cardiopulmonary Resuscitation
- Disease Models, Animal
- Heart Arrest
(therapy)
- Immunoenzyme Techniques
- Male
- Naltrexone
(analogs & derivatives, pharmacology)
- Neuroprotective Agents
(pharmacology)
- Piperazines
(pharmacology)
- Rats, Sprague-Dawley
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