Abstract |
Clear cell renal carcinoma (ccRCC) is the most common tumor of the kidney.in adults. The prognosis of ccRCC remains unsatisfactory. Recently, T cell immunoglobulin mucin-3 (TIM-3), a novel transmembrane protein, has been implicated in tumor biology. Its role in ccRCC remains unknown. The aim of this study was to investigate the roles of TIM-3 as a prognostic marker in patients with ccRCC.TIM-3 protein expression was determined by immunohistochemistry and Western blot from 137 ccRCC tumor samples and adjacent normal renal tissues. We performed also the cell proliferation assay using 3-(4,5-dimethylthiazol -2yl)-2,5-diphenyl-2H tetrazolium bromide (MTT) and cell invasion assay. The effects of small interfering RNA ( siRNA)-mediated knockdown of TIM-3 (TIM-3 siRNA) in two human ccRCC cell lines were evaluated. TIM-3 expression was higher in ccRCC tissue than in the adjacent normal renal tissue (P<0.001). High TIM-3 expression was an independent predictor of both cancer-specific survival and progression-free survival. TIM-3 protein was expressed in both ccRCC cell lines. Knockdown of TIM-3 suppressed the proliferation and invasion capacity of ccRCC cell lines.TIM-3 expression was associated with poor prognosis in ccRCC. Taken together, TIM-3 is a potential prognostic marker in ccRCC.
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Authors | J Yuan, B Jiang, H Zhao, Q Huang |
Journal | Neoplasma
(Neoplasma)
Vol. 61
Issue 1
Pg. 35-40
( 2014)
ISSN: 0028-2685 [Print] Slovakia |
PMID | 24195506
(Publication Type: Journal Article)
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Chemical References |
- HAVCR2 protein, human
- Hepatitis A Virus Cellular Receptor 2
- Membrane Proteins
- RNA, Small Interfering
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Topics |
- Adult
- Aged
- Carcinoma, Renal Cell
(chemistry, mortality, pathology)
- Cell Proliferation
- Female
- Hepatitis A Virus Cellular Receptor 2
- Humans
- Kidney Neoplasms
(chemistry, mortality, pathology)
- Male
- Membrane Proteins
(analysis, genetics, physiology)
- Middle Aged
- Neoplasm Invasiveness
- Prognosis
- RNA, Small Interfering
(genetics)
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