[Anti-tumor efficacy of P53 with 9R cell-penetrating peptides].

Abstract

To enhance the penetration of P53 into tumor cells by fusion it with the cell penetrating peptide 9R. The fusion gene of 9R-p53 was cloned into the expression vector. The fusion protein, CPPs-P53, was expressed and purified. We detected the rate of cell growth inhibition and apoptosis by MTT and Annexin-V-FITC/PI double stained method respectively for measuring its effect on tumor cells. CPPs-P53 and P53 were successfully expressed and purified, the purity of both proteins reached up to 90%. MTT assay showed that the cell growth inhibition by CPPs-P53 was more efficient than P53, and the rate of cell growth inhibition is dose-dependent. The apoptosis experiment showed that P53 could induce apoptosis of tumor cells. Compared with the P53, CPPs-P53 had a more significant effect in inducing cell apoptosis (**P < 0.01). The CPPs-P53 shows more significant effects than P53 in inhibiting cell growth and inducing apoptosis on tumor cells.

• Authors: Yuan Liu, Rui Chen, Nan Zhang, Xianlong Ye, Yin Bai, Yuquan Wei, Guiping Ren, Deshan Li
• Journal: Sheng wu gong cheng xue bao = Chinese journal of biotechnology (Sheng Wu Gong Cheng Xue Bao) Vol. 29 Issue 7 Pg. 955-64 (Jul 2013) ISSN: 1000-3061 [Print] China
• PMID: 24195362 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cell-Penetrating Peptides
• Tumor Suppressor Protein p53

Topics

• Apoptosis
• Cell Line, Tumor
• Cell-Penetrating Peptides (pharmacology)
• Humans
• Tumor Suppressor Protein p53 (pharmacology)