Cholera toxin enhances vaccine-induced protection against Mycobacterium tuberculosis challenge in mice.

Abstract

Interleukin (IL)-17 is emerging as an important cytokine in vaccine-induced protection against tuberculosis disease in animal models. Here we show that compared to parenteral delivery, BCG delivered mucosally enhances cytokine production, including interferon gamma and IL-17, in the lungs. Furthermore, we find that cholera toxin, delivered mucosally along with BCG, further enhances IL-17 production by CD4(+) T cells over mucosal BCG alone both in the lungs and systemically. This boosting effect of CT is also observed using a vaccine regimen of BCG followed by the candidate vaccine MVA85A. Using a murine Mycobacterium tuberculosis (M.tb) aerosol challenge model, we demonstrate the ability of cholera toxin delivered at the time of a priming BCG vaccination to improve protection against tuberculosis disease in a manner at least partially dependent on the observed increase in IL-17. This observed increase in IL-17 in the lungs has no adverse effect on lung pathology following M.tb challenge, indicating that IL-17 can safely be boosted in murine lungs in a vaccine/M.tb challenge setting.

• Authors: Kristin L Griffiths, Elena Stylianou, Hazel C Poyntz, Gareth J Betts, Helen A Fletcher, Helen McShane
• Journal: PloS one (PLoS One) Vol. 8 Issue 10 Pg. e78312 ( 2013) ISSN: 1932-6203 [Electronic] United States
• PMID: 24194918 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• BCG Vaccine
• Interleukin-17
• Cholera Toxin

Topics

• Analysis of Variance
• Animals
• BCG Vaccine (immunology, pharmacology)
• Cholera Toxin (pharmacology)
• Interleukin-17 (immunology)
• Mice
• Mycobacterium tuberculosis (immunology)
• Statistics, Nonparametric
• Tuberculosis (immunology, prevention & control)