Abstract |
The glaucophyte Cyanophora paradoxa (Cp) was chemically investigated to identify pigments efficiently inhibiting malignant melanoma, mammary carcinoma and lung adenocarcinoma cells growth. Cp water and ethanol extracts significantly inhibited the growth of the three cancer cell lines in vitro, at 100 µg · mL(-1). Flash chromatography of the Cp ethanol extract, devoid of c-phycocyanin and allophycocyanin, enabled the collection of eight fractions, four of which strongly inhibited cancer cells growth at 100 µg · mL(-1). Particularly, two fractions inhibited more than 90% of the melanoma cells growth, one inducing apoptosis in the three cancer cells lines. The detailed analysis of Cp pigment composition resulted in the discrimination of 17 molecules, ten of which were unequivocally identified by high resolution mass spectrometry. Pheophorbide a, β- cryptoxanthin and zeaxanthin were the three main pigments or derivatives responsible for the strong cytotoxicity of Cp fractions in cancer cells. These data point to Cyanophora paradoxa as a new microalgal source to purify potent anticancer pigments, and demonstrate for the first time the strong antiproliferative activity of zeaxanthin and β- cryptoxanthin in melanoma cells.
|
Authors | Paul-Hubert Baudelet, Anne-Laure Gagez, Jean-Baptiste Bérard, Camille Juin, Nicolas Bridiau, Raymond Kaas, Valérie Thiéry, Jean-Paul Cadoret, Laurent Picot |
Journal | Marine drugs
(Mar Drugs)
Vol. 11
Issue 11
Pg. 4390-406
(Nov 01 2013)
ISSN: 1660-3397 [Electronic] Switzerland |
PMID | 24189278
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Antineoplastic Agents
- Cryptoxanthins
- Pigments, Biological
- Xanthophylls
- Zeaxanthins
|
Topics |
- Antineoplastic Agents
(chemistry, pharmacology)
- Apoptosis
(drug effects)
- Breast Neoplasms
(drug therapy)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Cryptoxanthins
- Cyanophora
(chemistry, metabolism)
- Female
- Humans
- Lung Neoplasms
(drug therapy)
- MCF-7 Cells
- Melanoma
(drug therapy)
- Pigments, Biological
(chemistry, pharmacology)
- Skin Neoplasms
- Xanthophylls
(chemistry, pharmacology)
- Zeaxanthins
|