Abstract | ETHNOPHARMACOLOGICAL RELEVANCE:
Xiao-Xu-Ming decoction (XXMD) has been used to treat stroke and other neurological diseases for more than 1000 years. The purpose of this study was to investigate the effects of XXMD on mitochondrial damage and apoptosis after cerebral ischemia and reperfusion. MATERIALS AND METHODS: RESULTS: The results showed that XXMD treatment markedly attenuated ischemic changes, preserved mitochondrial integrity, and significantly reduced apoptosis. In addition, we found that XXMD treatment reduced p53 and Bax levels and increased Bcl-2 levels in mitochondrial fractions. XXMD significantly blocked the release of cytochrome c and Smac/Diablo from mitochondria, and inhibited activation of caspase 9 and caspase 3 in cytoplasmic fractions. Increased expression of c-IAP1 was observed in the XXMD60 group. CONCLUSIONS: The findings demonstrated that XXMD protected mitochondria from ischemic injury and inhibited apoptosis. The mitochondrial p53 pathway could be partially involved in the protective effects.
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Authors | Rui Lan, Yong Zhang, Jun Xiang, Wen Zhang, Guo-Hua Wang, Wen-Wei Li, Li-Li Xu, Ding-Fang Cai |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 151
Issue 1
Pg. 307-16
( 2014)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 24189031
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2013 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Drugs, Chinese Herbal
- Tumor Suppressor Protein p53
- xiao-xu-ming
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Topics |
- Animals
- Apoptosis
(drug effects)
- Brain Ischemia
(pathology)
- Drugs, Chinese Herbal
(pharmacology)
- Gene Expression Regulation
(drug effects)
- Male
- Mitochondria
(drug effects, metabolism, ultrastructure)
- Random Allocation
- Rats
- Rats, Sprague-Dawley
- Reperfusion Injury
(prevention & control)
- Tumor Suppressor Protein p53
(genetics, metabolism)
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