Abstract |
Polyclonal reagents have been used to define HLA class II molecules in conventional serologic and cellular typing. We generated human alloreactive T-cell clones to analyze the functional fine specificities of HLA class II molecules that might be important for the phenomenon of HLA and disease association. We chose to examine HLA-Dw14, an HLA-D specificity that has been associated with juvenile rheumatoid arthritis. In this paper we have presented data that suggest that conventional cellular typing does not reflect the distribution of T-cell epitopes on major histocompatibility complex class II molecules. We describe three alloreactive T-cell clones that have defined three separate Dw14-associated T-cell epitopes. Two of these epitopes were on a DR-region molecule; the third was located on a DQ-region product. In a panel of unrelated DR4-positive donors, these three DW14-associated determinants were present in a high frequency but were not linked to each other. Within the tested panel of DR4-positive cells, all possible combinatorial arrangements of these three allodeterminants were seen. The concurrent expression of any two of the three allodeterminants was equivalent to a positive typing response for Dw14. Our finding that HLA-Dw14 is not characterized by a unique allodeterminant but by the combinatorial recognition of independently distributed T-cell interaction sites suggests that analysis of HLA and disease association may be more clearly demonstrated through the use of human T-cell clones.
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Authors | C M Weyand, J Goronzy, C G Fathman |
Journal | Proceedings of the National Academy of Sciences of the United States of America
(Proc Natl Acad Sci U S A)
Vol. 83
Issue 3
Pg. 762-6
(Feb 1986)
ISSN: 0027-8424 [Print] United States |
PMID | 2418442
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antibodies, Monoclonal
- Epitopes
- HLA-DQ Antigens
- HLA-DR4 Antigen
- Histocompatibility Antigens Class II
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Topics |
- Antibodies, Monoclonal
(immunology)
- Cell Line
- Clone Cells
- Epitopes
(immunology)
- HLA-DQ Antigens
- HLA-DR4 Antigen
- Haploidy
- Histocompatibility Antigens Class II
(immunology)
- Humans
- T-Lymphocytes
(immunology)
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