Outbreaks of
measles and
mumps occur regularly in the UK. Rapid diagnosis of acute
infection is important for both infection control and epidemiological purposes. The objective of this study was to compare the performance of an automated platform (DiaSorin Liaison(®), Saluggia, Italy) with a manual capture
enzyme immunoassay (EIA; Microimmune, Hounslow, UK) for the detection of
measles and
mumps IgM antibodies in serum from symptomatic individuals. Ninety sera tested previously for
measles (n=50) and
mumps (n=40)
IgM using the manual EIA were tested retrospectively using the DiaSorin Liaison(®) and the results compared. Sensitivity, specificity, inter-assay variability and intra-assay variability of the Liaison(®) assays were calculated. Sensitivity and specificity of the Liaison(®) assay for
measles IgM were 92% and 100% respectively, with inter-assay variation of 14.1% and intra-assay variation of 12.5%. The sensitivity and specificity of the
mumps IgM Liaison(®) assay were 88% and 95% respectively, with an inter-assay and intra-assay variation of 13.9% and 5.3% respectively. Both the
measles and
mumps IgM Liaison(®) assays gave fewer equivocal results than the EIA. Neither Liaison(®)
IgM assay showed any cross-reactivity with sera positive against other viruses, however the
measles IgM EIA cross-reacted with parvovirus
IgM. The automated Liaison(®) assays are more specific, cheaper and less labour-intensive compared to the manual EIA. The Liaison(®) assays benefit from reduced number of equivocal results compared to the EIA for both
measles and
mumps IgM. This allows clinical decisions to be made accurately and in a timely manner.