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Rodent herpesvirus Peru encodes a secreted chemokine decoy receptor.

Abstract
Viruses have long been studied not only for their pathology and associated disease but also as model systems for understanding cellular and immunological processes. Rodent herpesvirus Peru (RHVP) is a recently characterized rhadinovirus related to murine gammaherpesvirus 68 (MHV68) and Kaposi's sarcoma-associated herpesvirus (KSHV) that establishes acute and latent infection in laboratory mice. RHVP encodes numerous unique proteins that we hypothesize might facilitate host immune evasion during infection. We report here that open reading frame (ORF) R17 encodes a high-affinity chemokine binding protein that broadly recognizes human and murine CC and C chemokines. The interaction of R17 with chemokines is generally characterized by rapid association kinetics, and in the case of CCL3, CCL4, CCL5, CCL24, and XCL1, extremely stable complexes are formed. Functionally, R17 potently inhibited CCL2-driven chemotaxis of the human monocytic cell line THP-1, CCL3-driven chemotaxis of peripheral blood mononuclear cells, and CCL2-mediated calcium flux. Our studies also reveal that R17 binds to glycosaminoglycans (GAGs) in a process dependent upon two BBXB motifs and that chemokine and GAG binding can occur simultaneously at distinct sites. Collectively, these studies suggest that R17 may play a role in RHVP immune evasion through the targeted sabotage of chemokine-mediated immune surveillance.
AuthorsOlga Y Lubman, Marina Cella, Xinxin Wang, Kristen Monte, Deborah J Lenschow, Yina H Huang, Daved H Fremont
JournalJournal of virology (J Virol) Vol. 88 Issue 1 Pg. 538-46 (Jan 2014) ISSN: 1098-5514 [Electronic] United States
PMID24173234 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
Chemical References
  • Chemokines
  • Receptors, Chemokine
  • Calcium
Topics
  • Animals
  • Calcium (metabolism)
  • Chemokines (metabolism)
  • Chemotaxis, Leukocyte
  • HEK293 Cells
  • Humans
  • Mice
  • Open Reading Frames
  • Receptors, Chemokine (genetics, metabolism)
  • Rhadinovirus (genetics)
  • Surface Plasmon Resonance

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