Abstract | BACKGROUND: OBJECTIVE: This double-blind randomized crossover study compared the impact of 4 wk of supplementation with 20 g MCT oil/d or 20 g corn oil/d on the kinetics of apolipoprotein (apo) B-48-containing TRLs and apo B-100-containing very-low-density lipoprotein (VLDL), as well as on the expression of key intestinal genes involved in lipid metabolism in 28 obese, insulin-resistant men. DESIGN: The in vivo kinetics of TRL apo B-48 and VLDL apo B-100 were assessed by using a primed-constant infusion of l-[5,5,5-d3] leucine for 12 h in the fed state. Real-time polymerase chain reaction quantification was performed on duodenal biopsy samples taken at the end of each phase of supplementation. RESULTS: Compared with corn oil, MCT supplements had no significant effect on plasma lipoprotein profile or TRL apo B-48 and VLDL apo B-100 kinetics. Positive correlations were observed between the intestinal expression of several key genes involved in lipoprotein metabolism in a subgroup of participants (n = 16) after MCT supplementation. However, there was no difference between MCT and the corn oil control supplement in the intestinal messenger RNA expression levels of these key genes. CONCLUSION: These data indicate that short-term supplementation with MCT has a neutral effect on TRL apo B-48 and VLDL apo B-100 kinetics and on the intestinal expression of genes involved in lipid and fatty acid metabolism in men with insulin resistance. This trial was registered at www.clinicaltrials.gov as NCT01806142.
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Authors | André J Tremblay, Benoît Lamarche, Marie-Ève Labonté, Marie-Claude Lépine, Valéry Lemelin, Patrick Couture |
Journal | The American journal of clinical nutrition
(Am J Clin Nutr)
Vol. 99
Issue 1
Pg. 54-61
(Jan 2014)
ISSN: 1938-3207 [Electronic] United States |
PMID | 24172309
(Publication Type: Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)
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Chemical References |
- Apolipoprotein B-100
- Apolipoprotein B-48
- Lipoproteins, VLDL
- RNA, Messenger
- Triglycerides
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Topics |
- Adult
- Apolipoprotein B-100
(blood)
- Apolipoprotein B-48
(blood)
- Cross-Over Studies
- Diet
- Dietary Supplements
- Double-Blind Method
- Humans
- Hypertriglyceridemia
(blood, complications, drug therapy)
- Insulin Resistance
- Intestinal Mucosa
(metabolism)
- Intestines
(drug effects)
- Lipid Metabolism
(drug effects)
- Lipoproteins, VLDL
(blood)
- Male
- Middle Aged
- Obesity
(blood)
- Pancreatitis
(blood, etiology)
- RNA, Messenger
(genetics, metabolism)
- Real-Time Polymerase Chain Reaction
- Triglycerides
(administration & dosage, blood)
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